464 Trypanosoma leivisi 
In the period of chronic infection, two nuclei may be observed in 
one cell (Diagram IV) sometimes united by a thin chromatic filament 
(fig. 1). One of the two nuclei is normal in structure, the other is 
always pycnotic and looks degenerated. This nuclear division is never 
followed by that of the cell, but it might be a reduction previous to the 
formation of gametes. Apart from these forms, we find others, where 
also a nuclear division takes place but where now two of them are 
obviousl)^ degenerated and fragmented (fig. 3), consequently I believe 
that these nuclear divisions are abnormal and to be regarded as 
pathological fragmentations. 
(e) Chromidia. The study of the formation of chromidia is easier 
under abnormal than under normal conditions, consequently the subject 
will be more fully dealt with when treating of the abnormal morphology 
of T. lewisi. Flagellates containing chromatic granules may be frequently 
observed in rats’ blood (Diagram V). Fig. 1 seems to indicate that 
Diagram V. 
Figs. 1—5. Different stages of the formation of chromidia. 
the chi’omidia may be formed under the influence of the nucleus and 
the blepharoplast. In fig. 4 the two forms of chromidia are yet to be 
separated, although they have become more diffuse; in fig. 5 they have 
intermixed. Figs. 2 and 3 show an agglomeration of the chromatic 
substance, leading to the formation of one or few compact chromatic 
masses, sometimes resembling a nucleus (fig. 3). These figures are 
interesting because similar stages have been described as an autogamy 
by Moore, Breiul and Kindle, the blepharoplast emitting chromatin 
