N. H. SWELLENGREBBL 
473 
I have seen similar changes, only the nuclei of my preparations do 
not possess such large karyosomes as Schilling has drawn. Minchin 
(1910) and I (1910) have shown elsewhere that these karyosomes are 
artefacts; Diagram X, fig. 4 shows the beginning of the autogamy, 
Diagram VII, fig. 2, Diagram X, fig. 1 and Diagram XII the subsequent 
stages. They can all of them be explained more naturally by super¬ 
position. So it is in Schilling’s figures, except fig. 2, but there it is by 
no means proved that the intranuclear granule near the karyosome has 
anything to do with the blepharoplast. I think that the existence of 
autogamy is not demonstrated by Schilling’s observation. 
3. Abnormal morphology. 
It is known that by preserving blood containing T. leiuisi in the 
refrigerator, the trypanosomes may survive for a considerable time (up 
to 20 days). During this time they become agglomerated, the proto¬ 
plasm becomes granulated and at last they degenerate completely. 
No attempt has been made to study systematically the different morbid 
changes occurring in trypanosomes under these circumstances, although 
this study is important so as to be able to interpret correctly the 
different forms found in the gut of invertebrate hosts (lice, fleas, bugs, 
ticks). In the paper by Strickland and myself (1910) we showed how 
many abnormal forms are found in the flea’s gut, etc. shortly after the 
infecting meal, before the real development begins. These forms have 
been interpreted by some authors {e.g. Prowazek) as sexual forms. I 
will try to show that similar forms are to be found in the blood pre¬ 
served in the refrigerator, so that probably the corresponding forms of 
the invertebrate hosts must be regarded as degenerative. 
The blood was kept for 7—11 days in the refrigerator in capillary 
tubes (mixed with a 0‘5 “/o solution of citrate of sodium in 0'5 saline). 
After 11 days the changes to be described here were well marked, after 
that time all the trypanosomes died and were dissolved except the 
flagella, so my description refers mainly to forms preserved for 11 days 
in the refrigerator. The different changes mentioned here occur all 
ante-mortem. 
(a) Changes of the hlepharojolast. (Diagram XIII.) The blepharo¬ 
plast becomes hypertrophied, sometimes the achromatic substance 
seems to free itself from the chromatic part (fig. 3), the latter may be 
divided in several portions (fig. 2). Also the achromatic part can 
become much elongated and curved, the chromatin distributed over it 
