258 
Agrippma hona 
coverslip the sporulation was only rarely iuteiTupted, and then by 
plasmolysis, owing to gradual concentration of the salt solution in v.’hich 
the cysts were immersed. 
In this paper, the process of gametogenesis more usual in gregarines 
will alone be dealt with. If opportunity arises in the winter, I hope to 
be able to discover if chromidiation reall}^ occurs in Agrippina as a 
normal process. 
Previous work on A. bona. 
Strickland has observed and figured the external form of spore, 
sporozoite, trophozoite, sporont and cyst. He conceives the karyosome 
of the trophozoite to consist of a closely wound skein, which later forms 
one or more rods or bands of chromatin. In the nucleus of the 
trophozoite and of the sporont, he finds spherical .structures which he 
calls “ polar bodies,” which do not take up the nuclear stain. As will 
be seen later, to call these bodies achromatic is rather to misrepresent 
their staining reactions. 
It is, however, Strickland’s description of what occurs in the cyst 
which is most remarkable, and passages in his paper referring to the 
nucleus and to the spores are here quoted:— 
“.the following nuclear changes can be made out. At first, while 
the two sporonts forming the cyst are still distinct, the nucleus of each 
half can be readily seen, but later, when the partition has disappeared, 
the nucleus cannot be seen under any circumstances. However, while 
it can be made out, it is seen to grow enormously in size, and its 
ultimate disappearance may be due to its having become commensurate 
with the cell. Meanwhile it is invested with a very definite nuclear 
membrane. 
“ The most strikinsr change that has occurred in it is the loss of all 
its basic staining substance or chromatin, which cannot be seen in any 
form. The band-like riband of chromatin seen in the sporont has 
disappeared. The spherical bodies occurring in the nucleus of the 
sporont are now very numerous; the nucleus contains many of them 
irregularly scattered through the ‘ karyolymph,’ and they may vary 
considerably in size. 
“ The details of the differentiation of the endoplasm into spores are 
very difficult to make out either in the living parasites or in stained 
preparations. Clear areas gradually loom up through the granular 
protoplasm, which is finally all used up, while the clear areas become 
