260 
Agrippina hona 
In young individuals the nucleus consists of a definite membrane 
containing karyolymph, in which occurs a fairly large karyosome. Tlie 
karyosome may contain refringent granules which are visible in vivo 
(Text-fig. 2, h, d). I find no evidence that the karyosome is really a closely 
wound skein, as Strickland describes. (PI. XVIII, fig. 1.) 
In the nuclei of the sporonts, the place of the karyosome is usually 
taken by a bent, irregular rod or baud, in which most of the chromatin 
is concentrated (Text-fig. 2, c). In nuclei of this stage there are 
usually present two (sometimes more) spherical bodies, called by 
Strickland “ polar bodies,” from their position. In preparations stained 
with Heideuhain’s ii’ou haematoxyliu, these spherules stain, sometimes 
very intensely. The older sporonts, whilst still retaining the remains of 
the band of chromatin (now usually in fragments), have their most 
intensely staining material concentrated in the spherules, which appear 
much darker than the chromatin band. In fact, in measure as the 
chromatin band stains less and less darkly, the more intensely do the 
spherules take up the haematoxyliu. A series illustrating this is given 
in PI. XVIII, figs. 2-5. 
With Giemsa’s stain (wet method) the chromatin takes on a deep 
purple Ime, whilst the spherules may stain a delicate rose pink, or may 
approximate to the chromatin coloration. It is difficult to obtain uniform 
results with this stain, but it seems probable that the spherules while 
yet achi’omatic stain pink, and later, when they contain cliromatin, stain 
purple. 
Altogether, it seems a fairly safe assumption that there is a gradual 
transference of chromatic material from the band to the spherules. The 
whole of the chromatin is, however, never transferred to the spherules; 
I’ods or granules always remain in the karyolymph, though they may 
stain but faintly. 
The Cyst. 
Sections of very young cysts show clearly that association of the 
sporonts must occur “ head to head,” and not “ tail to tail ” as Strickland 
suggests. 
This is evident from the persistence of the protomerites, which form, 
in longitudinal sections of young cj^sts, an equatorial body of lenticular 
sha.pe, lying symmetrically about the boundary between the two 
associated individuals (PI. XVIII, figs. 7, 8). This seems to be the stage 
at which staining material is most concentrated in the intranuclear 
spherules. 
