272 Rinderpest 
for a few moments only, accentuate the Methylene blue contours; but 
a ^ “/o solution must not be allowed to act longer than two or three seconds. 
The various methylene blue-and-eosin compounds (Giemsa, Romanowsky, 
Leishman), owing to the alcohol, all dissolve out the blue, and do not 
replace it. The bodies are thus washed out. Carmine and Iron Haema- 
toxylin are equally useless. For a long while the writer had been un¬ 
successful in the search for a means of staining more than the margins 
of the bodies ; hut the following procedure reveals the surfaces lying 
between these margins. The specimen having had any excess of the 
mercury salt and of blue washed off, is soaked for five minutes in the 
original blue solution, and overstained. Examined at this stage 
the corpu.scles are of a dense blue, and the bodies within them invisible. 
Wash, and stain in a mixture of Orange G. 2 “/o, Anilin blue 1 ®/o and 
Oxalic acid 2 ®/o in water, for ten seconds only. The corpuscles and 
contents are rendered deep chocolate, the bodies still invisible. Wash, 
first witli water, then with Absolute Alcohol, then with Acid Alcohol, 
then again with water. The alcohol washings must be brief, as they 
rapidly remove the stain. The result, in successful specimens, is to 
leave the corpuscle green, with the specific body very clearly shown 
within, of a deep brown colour (PI. XIX, figs, b—e shows the form, 
the brown colour not being represented). 
As has already been mentioned, by no one of very many methods 
tried by the writer has it been found possible to demonstrate the bodies 
in ordinary dried films : the application of the stain to them while yet 
the corpuscle is unfixed, as in the citrated solutions, seems to be essential. 
Conclusions. 
1. The occurrence of a body of special, and within certain limits, 
uniform morphology has been demonstrated in the red corpuscles of 
animals affected with rinderpest. 
2. The movements of the body, and the evidence of its growth pari 
passu with the development of the disease, and above all its reproduction 
in animals in which it was not previously present, on the inoculation of 
material containing it, are evidence of its being a living and independent 
organism. 
3. Its detected presence (so far) only in animals which at the time 
have, or which probably have had rinderpest recently, and its entire 
absence from animals highly susceptible to the disease, but known not to 
have had, or to have been exposed to infection, affords a presumption 
