276 
OBSERVATIONS ON MAMMALIAN ERYTHROCYTES. 
By J. CHARLES JOHNSON, M.A., M.Sc., M.B. 
{From the Quick Laboratory, University of Cambridge.) 
This brief note is the result of a series of examinations of blood-films 
of various mammals and was suggested by Dr Braddon’s interesting 
paper, the manuscript of which I had an opportunity of reading while 
working in the Quick Laboratory, Cambridge k 
The object of the research was to observe the red blood corpuscles of 
as many animals as possible, both healthy and suffering from blood- 
disease, with a view to finding’ any appearances similar to those described 
by Braddon as specific to rinderpest. 
Material. Owing to the kindness of Prof. Nuttall the blood of several 
of the animals on which he is experimenting at the University farm was 
placed at my disposal. The following is a list of the sources from which 
blood was taken for observation. 
Normal 
Man 
Two dogs 
Two rabbits 
White rat 
Calf 
Earn 
A bnormal 
Calf with East Coast fever (Theileria parva) 
Three dogs in various stages of acute piroplasmosis (Piroplasma 
canis) 
Two dogs in different stages of chronic piroplasmosis (P. canis) 
Horse with African biliary fever (Nuttallia equi); “salted” 
animal 
Method of collecting. The ear was usually selected as the site of the 
blood-letting. After preliminary shaving and swabbing with spirit a vein 
was opened with a sterilised needle or scissors. 
The blood was at first collected in watch-o']asses containinof various 
c5 O 
quantities of the staining fluid used by Braddon in Ids research (methyl¬ 
ene blue *5 "/o and potassium citrate, 1 “/o)- The watch-glasses were 
1 Dr Braddon’s paper appears in this number of Parasitology. —Ed. 
