S. Hadwen 
289 
Protocols of experiments. 
Tick-transmlssion e.rperiment (14-25 iv. 1913). 
Lamb No. I. 
Day 
1 Put eleven ? s and two s s D. vennstus on lamb’s back. 
2 One ? fell off without becoming attached. 
3 Eleven ticks attached themselves, all hut one (on back of ear) attached 
within half-an-inch of the median line on either side. 
5 Lamb not drinking his milk as usual in the evening. 
G Lamb weak. 
7 Loss of coordination becoming marked as day progressed; at 4 p.m. could 
' not stand. Two gorged ¥ s dropped off. Temperature 103T° P. 
8 Total paralysis, except for head and neck; lamb feeds well notwithstanding; 
continually grinds his teeth. Temperature 103T° F. 
9 Paralysis continues. 
10 Temperature 103'2° F. Incontinence of urine since paralysis started; 
cannot stop flow if tapped or startled. Drinks milk well. 
11 Temperature 103'3° F. Observed ticks copulating on lamb. Paralysis 
continues. 
12 Lamb raised his head easily in the morning ; was much better in afternoon ; 
could stand for a moment when raised ; also had more or less control 
over his urine when startled. 
Autopsy. Lamb killed by bleeding. Organs apparently normal. Coverings 
of the brain congested ; a fibrinous exudate present in the ventricles. 
Attempts to transmit the disease by inoculation (1913). 
Lamb No. II. 
Date 
25 IV. 
28 IV. 
3 V. 
3-13 V, 
13 V. 
13 v.-l VI. 
Inoculated in three places (lumbar, cervical and occipital regions), with 
material from brain, spinal cord and cerebellum of Lamb No. I. 
Temperature rose gradually to 105'5-106° F. Lamb had soreness between 
shoulder blades; temperature probably due to the formation of pus. 
Abscess broke; lamb better. 
Lamb perfectly well. 
Lamb was reinoculated with mashed-up ticks (three gorged ¥ s and one s ) 
which were removed from Lamb No. III. 
Lamb perfectly well; no effects from inoculation. 
Tick-transmission e.rperiment (5-15 v. 1913). 
Lamb No. III. 
Day 
1 Put seven ¥ s and three s s D. vennstus on lamb’s back. 
2 Temperature 103-3° F. 
3 Temperature : M. 103° F., E. 103-4° F. 
