28 
Blood Parasites 
stain, it was necessary to examine each film carefully with an oil 
immersion, objective and ocular 2 (approximately a magnification 
of 500 diameters), and even then it is quite possible that some were 
overlooked. Each slide was however searched very thoroughly, an 
average of three to four hours per film being spent. 
Tbe body of this trypanosome is .somewhat fusiform, and is about 
3-^- times as long as it is broad. The greatest width is at a position 
a little posterior to the middle, from whence it tapers off nearly equally 
at each end. The protoplasm stains a faint reddish-blue colour with 
Giemsa. It is for the most part granular and contains some vacuoles. 
In some specimens there are numerous chromatoid granules and this is 
especially noticeable in the ti’ypanosomes found in films made from the 
heart and lungs. There were no indications of myonemes. (Plate II, 
fig. 3.) 
The centrosome stains a deep crimson colour, and it is usually round 
or sometimes rod-shaped. Its position, about 2/r from the extreme 
posterior extremity, is fairly constant. 
The flagellum seems to originate from the posterior end of the 
centrosome, and this is well seen when that structure is rod-shaped. It 
runs forwards obliquely cro.ssing the body about twice and terminates 
as the free Hagellum. 
One very noticeable thing in practically all these trypanosomes is 
that the outer limitations of the undulating membrane stain a deep 
red colour, and running below it, and crossing it from time to time, is 
another line less deeply stained red. Whenever these two cross the 
body they seem to combine to form a single broad baud, although it is 
not difficult even then to see which is the outermost or attached 
flagellum. Possibly these are mere folds in the periplast, but unlike 
somewhat similar structures described by Minchin in T. leivisi, they run 
more or less parallel. 
The nucleus is situated about the middle of the body, and is 
somewhat round or oval in shape, with its long axis lying transversely 
to the long axis of the trypanosome. It measures on an average 3'8 long 
and 2’4/x broad. Stained with Giemsa or Leishman’s stain it appears 
to consist of a dense collection of chromatin gi-anules, and sometimes 
shows some indication of division. 
The free flagellum is about one half the length of the body. Out of 
the 91 trypanosomes two only belonged to the laige type. 
The Large Trypanosome of the Thrush. In addition to the two 
found in conjunction with the small variety, I met with examples of 
