68 
Treatment hi Piroplasmosis 
injection tlie teinperatnre showed a marked rise in temperature. On the 
fifteenth day the animal was not feeding, respirations hurried, temperature 
105'5° F., mucous membranes pallid, parasites present in small numbers 
in the blood. On the sixteenth day animal still not feeding, looking 
very depressed, eyes sunken, urine wine-coloured, temperature 106° F., 
parasites more numerous in the blood. 200 c.c. of a 2“/o solution of 
Trypanblue injected intravenously. Next day the temperature was 
down to 104° F., the animal began eating a little, looked brighter, and 
the parasites had greatly decreased in number and size. On the 
following day the animal had apparently quite recovered, temperature 
102’8° F., eating well, mucous membranes bluish, no parasites found in 
the blood. The animal made an uninterrupted recovery. 
General Conclusions on Practical Use of Trypanblue 
in Piroplasmosis. 
(a) Parasites are not always present in the peripheral blood of 
dogs, and are often extremely difificult to find in other animals, 
especially under conditions encountered in the field. If well marked 
clinical symptoms are shown one is justified in using Trypanblue, even 
if parasites cannot be satisfactorily demonstrated. 
(b) A large dose of a weak solution of Trypanblue should be 
administered. The writer cannot help thinking that the failures 
attributed to the drug by other observers are due, in many cases, to 
too small a quantity of the solution being injected. 
(c) After an experience of the treatment in some hundreds of 
cases, the writer feels justified in stating that animals do not suffer 
from relapses if a sufficient quantity of the solution is used. 
(d) The dog can become infected a second time after Trypanblue 
treatment, but this second infection is also amenable to the drug. 
REFERENCES. 
Nuttall axd IIadwen (1909). The successful drug treatiiient of canine piro- 
plasrnosis together with observations upon the effect of drugs on Piroplasma 
cams. Parasitolugy, ii. 156-191, 1 Text-fig. 
- (1909). Further experiments upon the drug treatment of canine piroplasmosis. 
Parasitology, ii. 229-2.35. 
- (1909). The drug treatment of piroplasmosis in cattle. Parasitology, ii. 
236-266, 7 charts. 
Ndttall xVND Strickland (1912). On the occurrence of two species of parasites 
in equine “ Piroplasmosis ” or “ Biliary Fever.” Parasitology, v. 65-96, 1 pi. 
charts and figs. 
