J. F. Valladares 
91 
films that were examined on each of the six days. This animal re¬ 
covered after medicinal treatment, which consisted principally of 
quinine and salol and was discharged from the Hospital on 9th 
December 1912. 
It was ascertained that this animal had never been out of Madras 
during about four years since it had been in the possession of the present 
owner. A month or so before it became ill it was sent for a few days 
to a local stable and, on its return to its home, ticks were removed from 
various parts of the body, which were immediately destroyed. I saw 
ticks on several animals standing in this stable which I visited subse¬ 
quently. These ticks belonged to the species Hyalomma aegyptium. 
Frequently on animals admitted into the Veterinary Hospital for treat¬ 
ment from this stable, I discovered ticks belonging to the same species. 
Several of them were dissected but with negative results. 
Suhinoculation experiment: an aged W. Gelding was inoculated 
intravenously with blood (5 c.c.) from Case 8 with positive result. 
The parasites (P. cahalli), after an incubation of 12 days, were easily 
distinguished in the peripheral blood, ring forms and bigeminum 
forms were mainly encountered. No medicinal treatment was given 
and tbe animal died early on the morning of 7th December 1912. 
There wei’e the usual symptoms of piroplasmosis : depression, rise of 
temperature to 100° F., yellowness of the visible mucous membrane 
and petechiae on the membrana nictitans, unsteady gait with complete 
paraplegia the evening before death. It should be stated however that 
this animal showed slight loss of co-ordination in the hind quarters 
before inoculation. 
Autopsy : characteristic; general bile staining of the subcutaneous 
tissue; liver enlarged, weight 17^ lbs. ; spleen greatly enlarged, 
somewhat pulpy, weight 13 lbs.; heart enlarged, brownish in colour, 
weight 3 lbs. 
Second subinoculation experiment: the aged Waler Mare already 
referred to in which the inoculation of Nuttallia equi strain from Case 1 
had failed, was inoculated with 5 c.c. blood from Case 8 on 5th December 
1912, but showed no reaction. 
Case 9 (Piroplasmosis). This case was discovered at the Madras 
Veterinary College Hospital in a Ch. W. Gelding belonging to 
Mr C. Morrison of Madras. This animal was admitted on 22nd 
December 1912, for a capped elbow. On 9th January 1913 it had an 
attack of colic from which it recovered. On 14th January the temper¬ 
ature rose to 105° F., and blood examination revealed an infection of 
