H. SeideltN 09 
3. Passage through xylol, and alcohols of diminishing strengths to 
distilled, or tap water. 
4. Staining for 5-10 minutes in a mixture of 3 pts. Solution A, 
and 2 pts. Solution B, which has been prepared not less than 15 minutes 
and not more than 2 hours. Sometimes equal parts of A and B may 
he used. 
5. Washing in tap water for 5 minutes or more. 
6. Passage through alcohols of increasing strengths to absolute 
and then xylol. 
7. Canada-balsam or Damarlack. 
II. Films. 
The only differences are, that these are not brought up to xylol 
after hardening, but passed from 90 “/o alcohol to water, and that the 
mixture of 3 A + 2 B is the only one that gives good results. 
After the staining they are treated exactly like sections, never 
being allowed to dry. 
Remarks on the Giemsa stain. 
The Romanowsky-stain has always been very difficult to apply to 
sections, in all its different modifications. Leishman’s method gives 
fairly good results, but it is a great drawback that, for obtaining them, 
it is always necessary to be in possession of fresh serum (1904). Also 
Giemsa’s method was originally intended only for smears which had 
been dried and fixed. But fortunately it now gives, when the principles 
laid down by Giemsa in his later publications (1910, 1 and 2) are 
strictly adhered to, excellent results also after wet fixation of films, and 
when applied to sections. In details some slight modifications may be 
made, whereby still better results may, in my experience, be obtained. 
As the method, which should certainly prove extremely useful, strangely 
enough does not seem to have come into general use, I shall describe 
the technique as I have now been using it on different materials for 
a considerable length of time. In fact, I had employed the acetone- 
differentiation a long time before the appearance of Giemsa’s papers on 
that subject, but I only obtained the very best results after having 
become acquainted with the elaborate details, with which the author 
himself describes the metho'd. Therefore the reader also must be 
Parasitology iv 
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