102 
Iron-haematein Stain 
4. Water, to which Lugol’s iodine-solution has been added in the 
proportion of 2 to 100, 5-10 minutes. 
5. Water. 
6. 0'5 7o solution of sodium thiosulphate, 5-10 minutes. 
7. Tap water, 5 minutes or more. 
8. Diluted Giemsa-solution 1 r 20 in distilled water, which has 
been neutralised (haematoxylin-indicatoz') with potassium carbonate 
solution, 1 hour. 
9. The same 1 : 40, 20-24 hours. 
10. Wash in tap water. 
11. Differentiate in pure acetone. (Control under microscope.) 
12. Acetone 70, xylol 30, 5 minutes. 
13. Acetone 30, xylol 70, 5 minutes. 
14. xylol, 5 minutes. 
15. Fresh xylol. 
16. Damarlack. 
I wish to express my thanks to Prof. E. A. Minchin for his kind 
permission to work in his laboratory, and to Dr H. M. Woodcock and 
Miss Muriel Robertson for material and for having tried the stain. I 
am also indebted to Dr J. W. W. Stephens for some material. 
REFERENCES. 
Giemsa, G. (1910). Ueber die Farbung von Schnitten mittelst Azur-Eosin. Deutsche 
med. Wochenschr. No. 12, p. 550. 
-Zur Farbung von Feuchtpraparaten und Schnitten mit der Azureosin-methode. 
Centralhl. f. Baktenol. u. Parasitenk. Abt. 1, Grig. Vol. liv. p. 489. 
Hansen, Fr. C. C. (1898). Eine zuverlassige Bindegeweb-sfairbimg. Anat. Am. 
Vol. XV. p. 151. 
Heidenhain, M. (1896). Noch einmal liber die Darstellung der Centralkorper etc. 
Zeitschr.f. wissenschaftl. Mikr. Vol. xiii. p. 186. 
Leishman, W. B. (1904). A method of producing chromatin-staining in sections. 
Joimi. of Hygiene, Vol. iv. p. 434. 
Minchin, E. A. (1909). The structure of Trypanosoma lewisi in relation to Micro- 
scoincal Technique. Qv,art. Journ. Micr. Sei. Vol. liii. p. 755. 
Rosenbusch, F. (1908). [Verhandl. d. deutschen tropenmedizinischen Gesellschaft.] 
Beihefte z. Arch.f. Schiffs- u. Tropen-Hygiene, Vol. xii. p. 277. 
Weigert, K. (1907). Eine kleine Verbesserung der Haematoxylin—van Gieson— 
Methode. Zeitschr. f. wissenschaftl. Mik. Vol. xxi. p. 1. 
