128 
Herpetomoiias ami Critliidia 
calliphorae a basal granule (at the base of the flagellum) and a marginal 
granule (at the point where the flagellum reaches the surface of the 
cell and becomes free) are present. The part of the flagellum situated 
betw^een these two granules is the rhizoplast. The marginal granule 
first divides; then follows the fission of the rhizoplast and basal 
granule; lastly, from the marginal granule a new flagellum grows out, 
so closely entangled with the old one that it often seems as if the 
production of the new flagellum occurs exclusively by fission. 
6. The extranuclear chromatoid granules of H. calliphorae consist 
of volutin. They are numerous during the preflagellate stage and 
disappear gradually during the flagellate stage; in the post-flagellate 
stage they are few in number or altogether absent. 
This behaviour suggests that volutin in H. calliphorae may act as 
a sort of nutritive reserve-substance. In degenerating individuals the 
volutin granules become extremely numerous, an abnormal condition 
observed also in Trypanosomata (“ volutinosis ”). Consequently volutin 
seems to play the double part of a nutritive substance, and product of 
degeneration; this, however, likewise holds for many other nutritive 
substances (fat, glycogen, etc.). 
7. The blepharoplast of H. calliphorae shows cyclical changes. 
During the formation of the post-flagellate stages the achromatic 
substance is lost and only the chromatic portion remains. The ble¬ 
pharoplast is smallest at this period. The preflagellate stages possess 
a larger blepharoplast, and this organellum reaches its maximum 
dimensions during the flagellate stage. I am not able to say whether 
or not these cyclical changes have any special importance; perhaps 
they have something to do with the regulation of the normal relation 
between nucleus and protoplasm. 
8. H. calliphorae was observed by me only in the gut of the fly 
and does not seem to be transmitted hereditarily, as is the case in 
Critliidia melophagia (Swingle, 1909; Porter, 1910). 
REFERENCES. 
Berliner (1909). Flagellatoiistudieu. Arch. f. Protistenk., xv. 297. 
Borowsky (1910). Untersuchungen iibcr xictinosphaerium eiclthonii. Arch. f. 
Protistenk., xix. 255. 
Calkins (1909). Protozoology. Loaand Febiger, New York and Philadelphia. 
Chatton and Alilaire (1908). Co-existence d’nn Leptomonas et d’un Trypanosoma 
chez un muscide non vulnerant. C. R. Soc. Biol., lxiv. 1004. 
