140 
Transmission of S. duttoni 
Exp. 7. O. moubata, adult t? , fed on infected mouse 7 days previously. Kept at a 
uniform temperature of 21° C. 
Tick material injected 
Result of 
inoculation 
Incubation 
period (days) 
Microscopical 
examination 
Gut + contents 
Malpighian tubules 
Salivary glands 
Testis 
Exp. 8. O. moubata, immature j , fed on infected mouse 7 months previously. Kept 
at a uniform temperature of 21° C. 
Tick material injected 
Result of 
inoculation 
Incubation 
period (days) 
Microscopical 
examination 
Gut + contents 
+ 
9 
- 
Malpighian tubules 
+ 
7 
- 
Salivary glands 
O- 
7 
- 
Testis 
-1- 
7 
- 
Exp. 9. O. moubata, immature ? , fed on infected mouse 4 months previously. Kept 
at a uniform temperature of 21° C. 
Result of Incubation Microscopical 
Tick material injected inoculation period (days) examination 
Gut + contents ' - - - 
Malpighian tubules - - - 
Salivary glands _ _ _ 
Ovary _ _ 
Exp. 10. 0. motibata, adult d , fed on infected mouse 4 months previously. Kept at 
a uniform temperature of 21° C. 
Result of 
Incubation 
Microscopical 
Tick material injected 
inoculation 
period (days) 
examination 
Gut + contents 
+ 
9 
- 
Malpighian tubules 
- 
- 
- 
Salivary glands 
- 
- 
- 
Testis 
+ 
7 
- 
Exp. 11. 0. moubata, adult 
¥ , fed on infected mouse 
4^ months previously. Kept i 
a uniform temperature of 21° C. 
Result of 
Incubation 
Microscopical 
Tick material injected 
inoculation 
period (days) 
examination 
Gut + contents 
+ 
8 
- 
Malpighian tubules 
+ 
8 
- 
Salivary glands 
- 
- 
- 
Ovary 
+ 
7 
- 
On summarizing the results described above, it will be seen that 
out of the eleven experiments, three were entirely negative, i.e., the 
ticks were immune to spirochaetal infection. 
The results of the eight positive experiments have been arranged in 
the form of a table, in which is given the total numbers of po.sitive 
