166 Fumigation of Ticks 
The following species of ticks were secured : 
Blue Tick, Boophilus decoloratus. 
Bont Tick, Amhlyomma hehraeum. 
Red-legged Tick, Rhipicephaliis evertsi. 
Brown Tick, Rhipicephalus appendiculatus. 
Black-pitted Tick, Rhipicephalus simus. 
Tampans, Ornithodorus moubata. 
The Blue Tick was secured in all stages—eggs, unengorged larvae, 
a few unengorged nymphs, unengorged and engorged females, and 
males. The Bont Ticks were secured as eggs, unengorged nymphs, 
engorged and unengorged females, and males. The Brown Ticks were 
all unengorged females and males. The remainder of the species were 
males and engorged and unengorged females. So far as possible the 
specimens were divided so as to have a few of each species and of each 
stage in each portion of the test. Check lots of the eggs used were 
kept apart to make sure of their vitality^ 
The tests were arranged so as to approximate as far as possible to 
the conditions which would exist in the hold of a cattle ship. 
One lot of ticks was placed in an open glass fruit jar with mosquito netting tied 
over the top so as to prevent the escape of the ticks but allow free entrance to the 
gas. This was then placed in the centre of the floor. With it were also placed the 
following preparations : ticks in a glass fruit jar with butter cloth tied over the 
top ; a fruit jar filled with a sheet of cotton wool in the middle of which the ticks 
had been jdaced and then rolled uji and tightly packed into the jar ; a paper shoe 
box containing ticks wrapped in three sheets of brown wrapping paper, one wrapped 
outside the other ; a glass fruit jar with ticks in the bottom and packed to the top 
with crumpled brown wrapping paper ; a fruit jar containing ticks and with 
mosquito netting tied over the top, but turned upside down on the floor ; a glass 
fruit jar, with ticks, over the top of which was tied a piece of bleached white calico ; 
and a fruit jar containing ticks but filled with crumpled brown wrapping paper and 
with a piece of butter cloth tied over the top. There were also prepared two pairs of 
boards about a foot and a half square. Between the first pair was packed hay to 
about a half-inch thickness, between the second jDair was packed about the same 
thickness of cotton wool. In the centre of the packing of each were placed the 
ticks, tied in a piece of mosquito netting to prevent their escape. Another lot of 
ticks was hidden in the centre of a bale of hay purchased on the market. Other 
lots were tied in butter cloth and sacking and hidden imder debris. One lot was 
placed in an old sack partly filled with hay. A box about one foot square by a foot 
and a half long was filled with dry sawdust and a lot of ticks tied in a piece of 
mosqviito netting hidden in the centre after which the cover was tied on. Other 
lots of ticks were placed in small glass honey jars, over the tops of which 
mosquito netting was tied, and hidden under coils of rope, sails and debris found 
