258 
Negri bodies 
(iii) Tetanus Toxin. In these experiments we were unable to find 
anything that resembled Negri bodies. The same number of tests 
were carried out with this toxin as with Russell’s viper venom. Sir 
David Semple, M.D., Director of the Central Research Institute, Kasauli, 
also kindly supplied us with material from guinea-pigs suffering from 
chronic tetanus. We were unable to confirm Poor’s observations on 
the bodies described by him as occurring in the nucleus. The only 
structures we saw in these nuclei other than true nucleoli, were the 
normal chromatin nodes. 
As the action of this toxin is exerted chiefly on the cells of the 
anterior horn of the spinal cord, we did not expect to see these Negri- 
like bodies in the hippocampus major or cerebellum ; nor did we do so. 
We did not consider it necessary to conduct experiments with strychnine, 
as this drug has a similar action to tetanus on the anterior cornual cells. 
(iv) Living Bacillus j)yocyaneiis emulsion. These experiments were 
conducted for a totally different purpose from that with which this 
paper is concerned, namely to. show that a septicaemia due to this 
bacillus occiu’S as a natural disease in dogs and that its symptoms 
closely simulate rabies. This work is to be shortly published by us. 
In the experiments that were performed we were able in two cases to 
find Negri-like bodies; these were found in the rabbit and were quite 
typical of Negri bodies as seen in these animals. 
(v) Normal cerebral matter of a dog was preserved for 72 hours in 
glycerine and then made into a thick emulsion; 1 c.c. was injected into 
the neck muscles of a guinea-pig. Fifteen animals were experimented 
with and fourteen of these died; one has survived up to date. In five 
of them control cultures were made from the heart blood, brain and 
liver and all these remained sterile. 
In one case bodies were seen resembling Negri bodies, but they 
were few in number and.only seen in the fascia dentata. In another 
case nucleolar fragmentation and extrusion of the fragmented particles 
were seen, but the particles still retained the nucleolar staining 
reaction. The other guinea-pigs did not show anything abnormal. 
A chameleon was given 1 c.c. of 1-100 living fixed virus emulsion. 
It survived for over a month and during the whole of this time 
apparently did not eat any food offered to it. On examination 
numerous large undoubted Negri bodies were seen in the cortical cells 
of the cerebrum. Whether these were due to the action of the fixed 
virus or not we cannot say. But in mammals fixed virus does not 
usually give rise to Negri bodies. 
