260 
Negri bodies 
nucleolus to be coloured by the counter-stains. These statements are 
based on an examination of a large number of sections—upwards of 
a thousand. With this explanation, it is possible for us to use the 
terms iron staining (black), Mann’s stain (red). Van Gieson’s stain 
(green and terracotta) without ambignity and so avoid a resort to 
the more common and at the same time more controvertible terms 
acidophile, basophile, cytoplasmic staining etc. 
(i) Man. We were fortunate in being able to examine six cases 
all told, three of which Major Cornwall, I.M.S., Director of the Pasteur 
Institute, Coonoor, kindly sent us. The Negri bodies seen were small 
in size, scanty in number, and for the most part occurred only in the 
large ganglion cells of the Ammon horn. They vary in size from 1-6 p. 
and appear homogeneous. Sometimes a vacuole is seen in them. As 
a I’ule these bodies in man have an affinity for Mann’s or Van Gieson’s 
stains. In the multipolar cells of the cortex, instead of Negri bodie.s, 
large irregularly shaped masses may be seen (PI. XI, fig. 2) lying 
usually at the base of the cells. They stain with difficulty and appear 
yellow with the Van Gieson mixture, and also with Mann’s stain. It 
seems likely that neither of the stains referred to is able to colour these 
masses. Examined under a high power, these masses appear to consist 
of an aggregation of small vesicles and sometimes they distend the 
nerve cell so that the nucleus may scarcely be discernible. The 
nucleolus of these cortical cells is single, and, by Mann’s method 
of staining, shows an outer red ring and a centre, probably fluid, which 
is yellow in colour like the extra-nuclear mass. These masses were 
first described by Major Cornwall, I.M.S. In a private communication 
we had from him lately he informs us that he does not now regard 
these masses as specific of rabies in man, because he has found them in 
diseased conditions other than rabies. 
(ii) Horse. The Negri bodies vary in size up to 10 /r, they are 
fairly numerous and sometimes contain a primary and even a secondary 
vacuole. Their staining reactions are the same as in man. In the 
cortex of a definitely rabid mule, similar yellow masses to those described 
by Major Cornwall, I.M.S., were found in the pyramidal cells. 
(iii) Bullock. The Negri bodies are very large and numerous and 
are seen in the cells of tlie hippocampus major, cortex and cerebellum 
(PI. XI, figs. 3 and 4). There are often several in a single cell and 
they vary in size from 4 p. to 20 /r. They are round or ovoid in shape. 
Frequently they are .situated near the nucleus, and, when they are in 
this region, they are indented by it. The small Negri bodies show a 
