262 
Negri bodies 
arrangement of primaiy and secondary vesicles is evident. With the 
Van Gieson mixture they usually stain greenish, but take the usual red 
colouration with Mann’s stain. The nucleus behaves, as regards its 
staining reactions, in precisely the same way. In the fascia dentata 
(PI. XI, fig. 7) the Negri bodies are about 2 to 6/r in length and many 
of them distinctly cap-shayjed ; on careful focussing the base of the 
cap is found to be indented by the nucleus. 
In the cerebellum they are usually large in size, irregularly vacuolated, 
and stain by Van Gieson. In the cortex they are numerous and scattered 
(PI. XI, fig. 8). The guinea-pig, goat and bullock are the only animals 
of this series, in which we have seen Negri bodies in the fascia dentata. 
(viii) Dog. The Negri bodies in this animal have often been 
described in detail. The only facts to be mentioned with regard to 
them are that they are often found in the dendritic processes of the 
nerve cells, and in this situation are elongated, showing that their 
contents are plastic. They usually stain terracotta but occasionally 
greenish with Van Gieson. The.nucleolus almost always stains greenish. 
(ix) Jackal. In this animal the Negri bodies closely simulate 
those seen in the dog as regards their size, shape, and staining reactions. 
They were more numerous, and the majority of them stained green 
with Van Gieson. We are disposed to believe that the younger Negri 
bodies in these various animals take the green stain and the older take 
the terracotta stain with Van Gieson. 
From the foregoing we see that these Negri bodies vary in shape, 
size, and staining reactions in the different animals examined, whilst 
remaining constant for the same animal. In the rabbit they are 
extremely small, 1 to 2 /a in size, non-vacuolated and stain terracotta 
(PI. XI, figs. 5, 6). In the bullock they are extremely large, sometimes 
as much as 20 /a, stain terracotta, and show vacuolation early in their 
development (PI. XI, fig. 4). In most of the animals examined they 
stained terracotta with the Van Gieson mixture, but particularly in the 
guinea-pig, though also in the jackal and dog, many of them stain green 
with this stain. The variations in the staining reaction of the nucleoli, 
as well as of these bodies, possibly denote a difference in chemical 
composition or reaction, but we state this with some reserve, as anyone 
who has worked on nucleolar structures knows the difficulty of inter¬ 
pretation of these reactions. 
In man, horse and mule the yellow masses, described by Major 
Cornwall, I.M.S., were seen; in the other animals examined they were 
absent. 
