300 
Oriental Sore 
fed were dissected at varying intervals after feeding. On no occasion 
was I successful in inducing a bug to feed twice from the sore. In all, 
twelve adult bugs fed on the sore, and became gorged with blood. Two of 
these were dissected twenty-four hours after feeding, eight were dissected 
forty-eiglit hours after feeding, and the remaining two seventy-two hours 
after. Developmental forms of the sore parasites were found in the 
stomach of one which was dissected twenty-four hours after feeding, in 
three wliich had fed forty-eight hours before, and in neither of those 
dissected seventy-two hours after. So that in four out of the total of 
twelve fed on the sore were flagellates found. 
Four young bugs which had hatched from eggs laid by bugs in the 
laboratory fed on the sure. Only three of these were dissected, all with 
a negative result. 
It has been mentioned above that seventy-two bugs taken from the 
prison and dissected immediately, gave no indication of a flagellate 
infection, so that there seems little doubt that the flagellates found in 
the gut of those fed on the sore represent cultural forms of the sore 
parasite. It is unfortunate that it was not found possible to conduct 
a greater number of experiments with the bugs hatched in the laboratory, 
but the greatest difficulty was experienced in persuading the young 
bugs to feed on the sore. The great majority of the many tided either 
persistently walked away or became involved in the exudate and there 
perished. 
The flagellate developmental forms of the sore parasite in the bed 
bugs are figured at PI. XII, figs. 1-21. It will be seen that they very 
closely resemble the cultural forms obtained on blood agar. Forty- 
eight hours after having been taken up, all are in one flagellate 
condition, while in the bug dissected twenty-four hours after feeding, 
rounded forms and clumps of incompletely developed parasites are found 
in addition to completely developed flagellates. In the bugs many 
abnormal forms are encountered, the protoplasm is often vacuolated and 
the nucleus broken up or absent. It seems probable that the develop¬ 
ment in the bug is an abortive one and takes place on account of the 
large quantity of blood which has acted as a good culture medium. 
These observations are very similar to those made by Patton on the 
development of the parasite of Kala azar in Cimex rotundatum. If the 
parasite of the sore can develop into a flagellate in the stomach of an 
insect, not its true host, then it would be expected that the very similar 
parasite of Kala azar would develop in the same manner. Such experi¬ 
ments and results only show that it is unsafe to draw the conclusion 
