302 
Oriental Sore 
proboscis was partly withdrawn and again inserted till the mosquito was 
satisfied with the supply of blood it was drawing. These mosquitoes 
more often prefer to feed on the thin red skin at the margin of the sore 
than on the healthy skin beyond. The rapidity with which the relatively 
enormous quantity of blood taken up at a single feed is digested 
and got rid of, is remarkable. In twenty-four hours the blown out 
Stegoviyia will have returned to its normal size and be ready for another 
feed. 
Stegomyia fed upon the sore and dissected immediately afterwards 
were found to have taken up the parasites in about 10 “/o of cases. In 
the others the parasites may have beeir too few for detection. In no 
instance were flagellates found in Stegomyia which had had only one 
feed on the sore. In one Stegomyia which had fed on the sore on two 
successive days and was dissected twenty-four hours after the last feed, 
there were found rounded forms of the parasite which resembled the 
enlarged forms seen in the early stages of the artiflcial cultures. 
In five other Stegomyia which had had a number of feeds varying 
from four to ten, and which had been dissected either twenty-four or 
forty-eight hours after the last feed, fully formed flagellates were found 
(PI. XII). 
In all, over eighty Stegomyia were fed in this way, the majority of 
these having had over five feeds and many of them ten. So that out of 
the eighty Stegomyia fed on the sore six were found to have evidence 
of a flagellate infection of the gut. This is a much smaller percentage 
than in the case of the bed bug, and here one can be less certain that 
one is dealing with developmental forms of the sore parasite and not 
with natural flagellates of the mosquito. In each case the hind gut 
was free from flagellates, a fact which is in favour of their being derived 
from the sore, since in natural infections the hind gut is generally the 
seat of the most intense infection. The experiments in nearly every 
instance were conducted with mosquitoes which had their first feed on 
the sore. 
The result is complicated by the presence of a Herpetomonas 
occasionally in the gut and malpighian tubes of the larvae. On no 
occasion however have these flagellates been found in the gut or 
malpighian tubes of the pupae, nor in the mosquitoes which had not 
fed on the sore though very many were dissected. 
As controls to the feeding experiments some six dozen Stegoviyia were 
allowed to have one or more feeds on a human being, but in none of 
these were any Herpetomonas found. So that the evidence is in favour 
