304 
Oriental Sore 
the pots the mosquitoes would sit on the sides, and of all methods for 
keeping them alive this was the best. There is constant moisture, 
a relatively low temperature, and a good supply of air. Of course, one 
has to be careful to keep the plate supplied with water, and further to 
keep all such jars out of the reach of ants which very soon destroy the 
flies and mosquitoes if they can attack them. House-flies and Stomoxys 
were kept in the same manner and also in wooden boxes with mosquito 
netting fronts. 
For actual feeding on the sore the mosquitoes or flies were liberated 
in a mosquito net and taken up separately in small glass tubes 
(3 X I inch). These were inverted open mouthed over the sore, and, 
after feeding, the mosquitoes or flies were returned to the respective 
jars. In the glass jars the mosquitoes survived much longer if a clean 
sterilised jar and cork were used each day. 
For breeding mosquitoes in the laboratory large mosquito nets 
were used and in these earthenware basins of water with the larvae were 
kept. Larvae were collected from the wells in small nets of mosquito 
netting fixed to the end of long bamboos. 
E. Character of the parasite as found in the sore. 
The parasite in smears from the sore may vary very much from the 
classical oval body with deeply staining rod and paler nucleus. The 
typical parasites are found in large numbers both within the large 
mononuclear cells which are probably of endothelial origin and also free 
in the plasma of the sore. In addition to these forms others occur in 
which there is considerable departure from the typical oval form. 
Dr Row has called attention to this fact and has figured many of these 
forms which are often much elongated and pointed at one end. In 
these elongated forms the kinetonucleus is often closely applied to the 
nucleus so that sometimes there is the appearance of the two having 
fused into one nucleus. In carefully stained films it is nearly always 
possible to distinguish the kinetonucleus even though it may be l 3 dng 
over the nucleus, and the number of forms where the two are in¬ 
distinguishable can easily be explained by the fact that the kineto¬ 
nucleus is under the nucleus and so rendered indistinct or invisible. A 
reference to PI. XII, figs. 22-24 will show these conditions. I do not 
think the kinetonucleus and nucleus have fused in any of these 
elongated forms as Dr Row thinks must be the case. Nor do I think 
