306 
Oriental Bore 
body. This appears to be part of the kinetonucleiis and not a separate 
structure like the blepharoplast of some trypanosomes. It is from the 
margin of this paler body that the rhizoplast takes origin, whence it 
runs to the surface of the body. The first indication of a preparation 
for division is the growing out from this pale body of a second rhizoplast, 
parallel to the first (PL XII, fig. 26). This new rhizoplast is at first 
thinner than the original one, but it gradually increases in thickness 
till it attains the same size. Such a connection of the flagellum to the 
kinetonucleus I have seen in some of the reproducing forms of Trypano¬ 
soma letvisi. Too much weight cannot be attached to the appearances 
presented in dried films, but a similar connection can sometimes be 
made out in films, prepared by more rational methods. The protoplasm 
of the parasite is usually vacuolated to a greater or less extent and 
finely granular. Some of the granules may stain red with Giemsa’s 
stain. The appearance of the nucleus in these dried films is well 
known and calls for no remarks beyond this, that the drying process has 
completely destroyed the normal appearance. 
In films fixed in Schaudinn’s fixative and stained by the iron 
haematoxylin method, the normal appearance is better retained. The 
parasites however are so minute, that it is difficult to make out clearly 
such details as the relation of the rhizoplast to the kinetonucleus. This 
is more clearly shown in some of the lar’ger cultural forms. However, 
in films from the sore fixed and stained thus, one sees clearly that the 
nucleus is a spherical body limited by what appears to be a delicate 
membrane. At the centre of the nucleus is the deeply staining 
karyosome which varies in size according to the extent of extraction of 
the stain by the iron alum solution. The rhizoplast is as a rule very 
difficult to detect in these preparations. The kinetonucleus stains as 
a black rod (PI. XIII, figs. 9 and 15). 
Examinations of the sores made to discover if any morphological 
change occurred in the parasite as the sore developed, showed that all 
the forms described above could be found in the youngest as Avell as in 
the oldest ones. In the older sores that are commerrcing to heal, the 
most noticeable feature is a diminution in the number of parasites which 
eventually become very difficult to find. 
In the non-ulcerating sores, the parasites occur in the large 
mononuclear cells. The nuclei of these cells are frequently riddled 
with parasites and may be completely destroyed by them. Portions 
of these cells are often broken off in the process of film making, and 
one has the appearance of several parasites embedded in an enucleate 
