308 
Oriental Sore 
G. Character of the developmental forms of the sore 
parasite in blood agar culture. 
The parasites in cultures may be studied in dried films stained by 
Giemsa’s stain, or better in films fixed without drying and stained 
by Heidenhain’s iron haematoxylin method. Some of the flagellate 
forms as seen in dried films are shown at PI. XII, figs. 30-36. In some of 
these the karyosome within the nucleus is shown in stages of division, 
but they are most interesting from the point of view of the flagellum 
which shows a connection with the kinetonucleus similar to that which 
was seen described above for the parasites from scrapings of the sore. 
In these it appears as if the kinetonucleus is a structure enclosed by 
a delicate membrane, on one side of which lies the deeply staining 
chromatin mass. From the opposite side of the membrane springs the 
flagellum, and it is from this membrane that a new flagellum grows out 
when division is about to take place. Sometimes the spot at which the 
flagellum unites with the possible membrane appears slightly enlarged 
and has the appearance of a blepharoplast. 
The structure of the cultural forms is very well shown in films 
stained with iron haematoxylin after fixation in Schaudinn’s fluid 
(PI. XIII). It will be seen that the resting nucleus like the nucleus of 
a trypanosome consists of a delicate membrane enclosing a clear space 
at the centre of which lies a deeply staining karyosome. The kineto¬ 
nucleus is a rod-shaped body staining black. The exact connection of 
the flagellum with this is difficult to trace. In some it appears to unite 
directly with the kinetonucleus (PI. XIII, figs. 1, 8, 10, 13). In others 
it cannot be traced so far, while again it may be united by a faintly 
staining structure (fig. 11) resembling to some extent the appearances 
given in the dried films. There does not seem to be a separate blepharo¬ 
plast as in many trypanosomes. Such a cone-shaped structure has been 
described and figured (figs. 4 and 5) by Robertson and Minchin in the 
collar cells of Glathrina coriacea, and I have shown a somewhat similar 
cone-shaped prolongation of the nuclear membrane in the case of 
Cercomonas in which the flagellum arises from the summit of the cone. 
The various division stages are very clearly shown in the wet fixed 
films, especially in some of the short stumpy forms. The first in¬ 
dication of a division is the further elongation of the already rod¬ 
shaped kinetonucleus and the formation of a second rhizoplast parallel 
to the first. The earliest stages of the formation of the new rhizoplast 
