318 Oriental Sore 
24. No treatment has had much effect in reducing the duration of 
the sore. 
25. Much can be done by protective inoculation on unexposed 
surfaces of the body not only to avoid the disfiguring scar on the face, 
but to prevent this having access to sores and thus becoming infective 
to other people. 
II. The Life History of Lankesteria culicis (Ross, 1898). 
Gregarine in Stegomyia fasciata. 
General account of the infection. Gregarines in this mosquito were 
first noticed and described by Ross during his classical researches into 
the development of malarial organisms on mosquitoes. The main 
features of the life history of this gregarine were described by him as 
follows. 
“ The youngest gregarines are found in the perivisceral cells of the 
youngest larvae. Growing in size, they escape from the host cell, 
become active and when the larva develops into a pupa, migrate into 
the malpighian tubes. There they become encysted with or without 
conjugation, and produce a large number of pseudonavicellae which 
are expelled with the faeces of the imago, either into water or upon 
the human skin.” This gregarine was subsequently rediscovered by 
Marchoux, Salimbeni and Simond when conducting investigations on 
yellow fever. It is evidently the same gregarine which is to be found 
in many of the Stegomyia fasciata of Bagdad. Here the Stegomyia 
breed chietiy in the wells, and I have noticed that not all the wells are 
infected with gregarines to the same extent. In some wells practically 
every larva or pupa is infected, while in others only a small percentage 
and in some the Stegomyia appear to be free. 
By a process of section cutting, somewhat laborious, of the body 
contents of the Stegomyia in all stages of its development I have been 
able to follow out almost the complete cycle of development of the 
gregarine. The body contents of larvae were dissected and placed one 
upon the other upon a slide kept moist under a glass cover, so as to 
form a small heap. When twenty to thirty had so been gathered into 
a heap the whole mass was fixed in Schaudinn’s fixative. There was 
little tendency for the individual parts to separate so that it was 
possible to treat the collected organs as a single piece of tissue. This 
was waslied, cleared and embedded in paraffin in the usual manner. 
The mass was then cut into serial sections which were stained with 
