322 
Oriental Sore 
present. In the former of these two gregarines the karyosome (fig. 30) 
with two vacuoles was lying free in the protoplasm. Though the 
nuclear multiplication had advanced as far as the third division, it was 
still intact. Attached to it at one side is a peculiar club-shaped body. 
A similar structure occurred in the nucleus of another gregarine 
(PI. XV, figs. 1 and 2). In subsequent divisions the definite character 
of these Spindles is lost or they are too minute to be detected readily. 
PI. XV, fig. 19 shows a section of a gregarine cyst where some of the 
nuclei of one gregarine are already arranged on the surface preparatory 
to gamete formation, while others presumably in the other gregarine 
are in division. In this nuclear division all that can be made out is the 
spindle-shaped structure without any astral system round the poles, or 
a visible centrosome. In the minute spindles the chromatin appears at 
first as a single mass at the centre. It divides into two, each half 
supplying the chromatin of a daughter nucleus. It is possible that 
even at this stage the centrosome, astral system and spindle fibres exist, 
but that they are too small to be clearly made out. As the nuclear 
divisions proceed some of the nuclei cease to have any share in the 
multiplication process and they remain as undividing nuclei in the 
protoplasm (PI. XV, fig. 19). They take no part in the formation of the 
gametes and ultimately degenerate with the residual protoplasm left 
over when the gametes are formed. During the process of nuclear 
multiplication the bodies of the gregarines, at first clearly distinguishable, 
became interlaced owing to their protoplasm becoming vacuolated and 
thrown into folds and ramifications. In such a cyst as that figured at 
PI. XV, fig. 19, it is impossible to trace the bodies of the two gregarines. 
Presumably the nuclei which are still dividing belong to one gregarine, 
while the others which have placed themselves on the surfaces of the rami¬ 
fications in preparation for the formation of gametes belong to the other. 
Gamete formation and conjugation. When nuclear division is com¬ 
plete, the nuclei arrange themselves on the surface of the ramifications 
into which the bodies of the gregarines have been produced. Pointed 
elevations of this surface are formed and into each bud a single nucleus 
enters. The buds are then separated as small gametes 3‘5-4;U, in 
diameter. The nucleus of each gamete is placed eccentrically and 
beyond the nucleus the protoplasm is produced into a pointed pro¬ 
tuberance (PI. XV, figs. 12, 14, 17). The protoplasm of the gametes 
shows a mai’ked reticular structure whereas the pointed eminence 
beyond the nucleus is quite clear and hyaline. It is possibly a con¬ 
densed portion of the thin limiting ectoplasmic layer. 
