336 
Oriental Soi'e 
This first divides into two equal parts (figs. 16 and 13). Each half then 
passes to one side of the kinetonucleus (figs. 9 and 10) and then the 
kinetonucleus itself is divided. Apparently the dark staining body 
which is within the kinetonucleus functions as a centrosome. The 
details of this division have been described but it must be mentioned 
that the films were dried ones stained by Giemsa stain. It is just 
possible that some of the appearances are the result of bad fixation, but 
they are so constant and occur in so many of the dividing flagellates 
that this can hardly be the case, though one has to exercise caution in 
drawing conclusions. After division of the kinetonucleus, division of 
the nucleus takes place, but it must be stated that the kinetonucleus 
does not divide till the new flagellum is equal or almost equal to the 
old one in length and thickness, so that many of the flagellates appear 
in the preparations with single nucleus and kinetonucleus, and two 
equal flagella which are bound together by some connecting material 
which is, according to my interpretation, the common flagellar sheath. 
These forms correspond exactly with Prowazek’s figures and many of 
them show clearly the granule or diplosome at the base of each flagellum 
(figs. 18,15,10, 20). Just before or at the time of division of the kineto¬ 
nucleus the common sheath which binds the two flagella together is 
divided longitudinally, so that the flagella may move independently of 
one another (fig. 9). Immediately after division of the kinetonucleus 
each blepharoplast divides again, a new rhizoplast is formed and a new 
flagellum grows out in such a way that these dividing flagellates have 
four flagella. This condition is very well shown in the flagellate drawn 
at fig. 14 and it corresponds very closely with some of Prowazek’s figures 
of this stage. 
Occasionally the formation of the new flagella may commence before 
the division of the kinetonucleus is completed, but this is a rare 
occurrence (fig. 9). The forms with four flagella are not numerous, for 
division of the two nuclei is quickly followed by division of the body of 
the flagellate. The result of this process of division is that none of the 
flagellates are uniflagellate, for at the time of division each half is pro¬ 
vided with one fully formed flagellum and one partially formed one 
(fig. 17). It thus comes about that during the active multiplication 
uniflagellate forms are not to be found, and I think that this fact has 
undoubtedly led to Prowazek’s interpretation of this flagellate as being 
a biflagellate. When, however, active multiplication is not in progress 
the flagellate is often found with a single flagellum. This is the resting 
form and every transition between the uniflagellate elongated forms 
