“>04 
fttegomyia fasciata 
After 24 hours: 
Increase in hatching 
No. 1, more eggs had hatched—probably all. 
^Xo. 2, 8 larvae out ... ... ... ... 6 
No. 3, 3 „ 1 
No. 4, 2 „ 1 
No. 5, 12 „ 12 
After 68 hours: 
No. 1, all the eggs hatched, larvae have grown rapidly. 
No. 2, 9 larvae out^—slight progress. 
No. 3, 4 „ no progress. 
No. 4, 2 „ both dead. 
No. 5, all (12) larvae out, have grown rapidly. 
The strength of the soda and ammonia solution in tubes Nos. 3 and 4 was 
doubled to 1 in 500. 
After 24 hours no further eggs had hatched. 
To each tube. Nos. 2, 3 and 4, -5 c.c. of a .culture of B. coH was added. 
One hour later: all the remaining eggs in tubes 2. 3 and 4 had hatched— 
No. 2, 12 or 13 living larvae. 
No. 3, 12 or 14 living larvae. 
No. 4, 12 living larvae. 
The larvae in No. 4 (ammonia 1 in 500) all died within 24 hours. 
Two days later the larvae in tubes Nos. 2 and 3 had made very rapid progress 
and had caught up to those in tube No. 5 in the matter of growth. They actually 
passed them during the next day or so and then slowed down. The larvae in all 
these tubes came to a standstill within 10 days, having apparently exhausted their 
food supply. 
Experiment V {unsterilized conditions). 
Ability of the larvae to develop on a diet of bacteria. 
Temperature 75° F. 
Bacillus cvli was washed by centrifuging 4 times in succession and a small 
quantity of the precipitate placed in 30 c.c. of tap water with 5 newly-hatched larvae. 
Control. 5 newly-hatched larvae of the same batch were placed in 30 c.c. of tap 
water, minus the B. coli. 
The control larvae marked time with little or no progress; 1 larva died on the 
6th day and 1 was feeble and died on the 8th day. By the 11th day 2 of the 
larvae passed the first moult, while a third larva died on the 12th day. After 17 
days 1 larva attained to its third instar; both the larvae were hving after the 
30th day, but were dead by the 37th day. The larvae given B. coli grew rapidly; 
by the 3rd day 2 of the larvae had passed the second moult and the others were 
nearly full grown in their second instar. 
^ It is probable that tube No. 2 was already infected as numerous later tests of B. coli 
filtrate added to tubes in which dormant eggs were lying, showed that the filtrate did not 
stimulate hatching. 
