506 
Stcgomyia fasciata 
After 4 clays the formation of gas ceased and the larvae again commenced to 
grow rapidly, and within 3 days some had attained their third skin. The tube, 
however, became very foul and all the larvae were killed. 
Experiment VIII. 
Eggs sterihzed by Method a were pipetted into a tube of peptone water and 
a tube of beef broth without either salt or peptone. 
No eggs hatched in either tube in response to the conditions and lapse of time, 
which were successful in the first trial. 
Broth. After 48 hours sterility broke down in the broth tube; many of the eggs 
hatched and the larvae made rapid progre.ss, 4 days later some of the larvae 
passed their third moult, but others were much behind on the 24th clay of the 
experiment; 22 days after hatching the first adult was reared and others followed, 
but there wtus a heavy death-rate, presumably owing to there being too gross a 
growth of bacteria in the tube. 
Peptone ivater. This tube remained sterile and, in spite of warming and cooling, 
no eggs hatched until the 3rd day, when one larva emerged; others followed and 
5 or 6 were .seen by the 5th day. Growth was not quite inhibited, but hardly any 
progress had been made by the 4th day after hatching, the larvae being still in the 
first instar. 
On the 8th day of the experiment, 4 days after the hatching of the eggs, sterility 
broke down, the tube becoming cloudy. The larvae emerged from the remaining 
unhatched eggs and rapid progress commenced. 
The larvae made rapid growth at first, and, owing to their number or the less 
vigorous growth of the bacteria, the conditions in the tube remained healthy, the 
larvae keeping the clouding of the peptone water in check and finally clearing it; 
they then began to find a shortage of food and ceased to grow, finally dying on the 
24th *day of the experiment—the last to die having reached the third instar. 
Experiment IX. 
(Includes notes respecting the addition of acid on dormant eggs.) 
Eggs were sterilized by Method b and pipetted into four tubes of peptone water 
and four tubes of broth, only 5 or 6 eggs being placed in each tube. 
After 48 hours, 4 eggs had hatched in one of the tubes of broth (No. 1) and in 
this tube only had sterihty broken down; a 5th larva emerged in this tube next 
day. A plate culture was made from this tube, and a sporing bacillus isolated. 
On the evening of the 3rd day from the start one of the broth (No. 2) and one of the 
peptone tubes (No. 3) in which no eggs had hatched, were inoculated from the tube 
which had become infected. By next morning 4 larvae had hatched in the broth 
and 1 in the peptone tube. None hatched within the next 2 or 3 days in the 
remaining sterile tubes. The following test was then made: to two of the sterile 
tubes—broth No. 4 and peptone No. 5—was added sufficient of a dilute solution 
of HCl to produce a similar acid reaction to the broth tube in which sterility broke 
down. No response took place within 20 hours, but the remaining eggs in the inocu¬ 
lated peptone water hatched within this period. 
By the 7th day of the experiment the larvae in the broth tube in which sterility 
broke down were all in the fourth instar, while those in the inoculated broth tube 
