508 
Steo’omvia fasciata 
o ». 
Tubes Nos. 4 and 5. Four days after hatching, the larvae had made good pro¬ 
gress, those in the broth tube being well ahead, while by the following day the growth 
of bacteria in the peptone water had become exceedingly dense and one or two of 
the larvae were dead. By the 7th day after hatching 4 larvae in the broth tube 
had pupated, while the surviving larvae in the peptone water were still in their 
third instar. Five days later (12 days from hatching) all the larvae in the peptone 
water were dead, and a pair of adults had emerged from the pupae in the broth 
tube; the remaining pupae and larvae died. 
Tube No. 6. These larvae made very slow progress; the growth of bacteria 
in the tube being feeble. By the 6th day after hatching they had only attained 
to their second instar. Five days later the larvae were still all living and had made 
steady progress; one pupated on the 13th day from hatching and 2 SS emerged 
on the 17th day; 2 following on the 18th and 20th days. 
Tube No. 7 (broth). Four days after hatching only 2 of the 4 larvae were living; 
these had made rapid progress and were in their fourth instar; 2 cJ specimens were 
bred on the 11th day from hatching. 
Experiment X. 
Washed B. coli killed hy heat as food for the larvae. 
A small quantity of washed B. coli was put into two tubes of distilled water 
which were sterilized by heat. 
Into one tube a larva was transferred from a tube of peptone water that had 
remained sterile for several days; into the other several larvae that had just emerged 
from unsterihzed eggs placed in tap water. The cloudiness which followed in this 
tube indicated that the larvae had infected it. The growth of these larvae was 
rapid; on the 5th day they were well grown in the third iastar—the sterile larvae 
having made barely appreciable progress in the first instar. 
The progress of the infected larvae slowed down gradually as the water cleared, 
but they continued healthy. 
In the sterile tube the larvae progressed very slowly and died after moulting 
on the 9th day. 
Experiment XI. 
(Includes notes concerning the effect of sterile yeast extracts on dormant eggs 
and a change of diet, under sterile conditions, on the larvae.) 
Five to 7 eggs sterilized by Method b were transferred by pipette into the follow¬ 
ing tubes: 4 of peptone broth; 3 of peptone water; 3 of beef broth (no salt or 
peptone); 1 in boiled tap water and 1 in boiled distilled water. 
After 24 hours eggs left in the lysol used in the sterilizing process had hatched; 
the larvae were dead and many had failed to get free of the eggshell. Only one 
egg hatched out of a number that were transferred from the lysol to tap water. 
Several eggs out of a large number left in the flask of boiled water hatched. Some 
eggs also hatched in the tubes of peptone broth, broth tubes, and in one of the pep¬ 
tone water tubes, but none in the boiled tap water or boiled distilled water. 
After 48 hours one larva hatched out in the boiled distilled water; none in the 
boiled tap water. 
