510 
Stegomyia fasciata 
Beef broth. In two tubes (Nos. 1 and 2), the larvae were growing steadily, if 
slowly; in No. 3 progress was very slow. 
On the 14th day: 
The larva which hatched in the boiled distilled water died in its first instar. 
Peptone water. One of the two larvae which hatched in the sterile tube was 
dead. In the B. coli infected tube the larvae had already passed the second moult 
(about 48 hours after hatching). In the third tube, which remained sterile, the eggs 
were still unhatched. 
Peptone broth. Tube (No. 3), one of the two larvae was dead, the other had 
moulted; (No. 2), 3 larvae were in the second and 1 in its third instar, all small 
and ill-grown. In tube No. 1 another larva had hatched, but one of those which 
had emerged previously was dead; of the living 2 were in the second and 2 in the 
first instar. In the infected tube (No. 4) the larvae were making slow but steady 
progress in their second and third instars. 
Beef broth. Tubes Nos. 1 and 2 had become infected and the larvae were pro¬ 
gressing. 
15th day: 
Distilled water. A second larva had emerged in this tube. 
Tap water. Eggs still dormant. 
Peptone water. In the B. coli infected tube all the larvae had reached their 
fourth instar and one had died. 
To the sterile tube in which no eggs had hatched -5 c.c. of sterile watery extract 
of brewers’ yeast was added. (Without result.) 
Peptone broth. Tube (No. 1), the two first instar larvae were taken to test if 
change ^ i a different media would affect growth. A note will be found at the end 
of this experiment (page 511), both the second instar larvae were dead; (Nos. 2 
and 3), no change; (No. 4), the infected tube, the larvae were making slow progress. 
Beef broth. The larvae in the infected tubes (Nos. 1 and 2) progress steadily; 
in No. 3 the progress is scarcely perceptible. 
19th day: 
Distilled water. The second larva which hatched was also dead. 
Tap water. None of the eggs had hatched. 
Peptone water. In the tube infected with B. coli aU the larvae reached full 
growth and then died. 
Of the larvae which hatched out in the sterile tube, 1 had died; the living ones 
made no progress. 
The eggs in the tube to which sterile yeast extract was added had not hatched. 
Peptone broth. (No. 1), no living larvae remained; (Nos. 2 and 3), the larvae 
all dead; (No. 4), growth was variable, the larvae which hatched prior to infection 
were ahead of the others. 
Beef broth. Larvae in the sterile tube were making very slow progress; in the 
infected tubes they were growing steadily, though not rapidly. 
22nd day: 
Distilled water and Tap water. No change. 
Peptone water. In the sterile tube the larveie made no progress. The yeast 
extract did not cause the eggs to hatch in the third tube; this tube was inoculated 
with a living yeast and the eggs hatched during the night. 
