E. E. Atkin and A. Baoot 
517 
No. 54, sterile; 2 larvae living; little if any change. 
No. 55, sterile; 2 larvae living; 1 in fourth and 1 in second instar. 
With charcoal. 
No. 57, the last larva is now dead. 
Evaporation had so reduced the quantity of fluid that boiled distilled water was 
added to tubes Nos. 51 , 52 , 54 and 55 . Following the addition of the water a few 
eggs that had lain dormant in tubes Nos. 51 , 54 and 55 hatched. 
Nine days later, 135 days from start. 
No. 51, it was found that all the larvae were dead. 
No. 52, 1 of the 4 larvae died. 
No. 54, only I of the 2 original larvae living; those that hatched out recently 
are dead. 
No. 55, the recently hatched larvae have disappeared, possibly swallowed by 
the larger ones. 
Tube No. 54 inoculated with a yeast (S. cerevisiae). 
142 days from start. 
No. 52, aU the old larvae are dead, the last two died with their jaws entangled 
in a small tuft of hair. The three survivors are growing steadily. (Sterile.) 
No. 54, this tube was inoculated with a yeast {S. cerevisiae ); the surviving 
larva after one week looks large and stout in comparison with its dead companions. 
No. 55, the surviving old larva is still active, two of those which hatched after 
the addition of fresh water are living and are now in second skin. (Sterile.) 
152 days from start. 
No. 52, moulds grew in the tube; the three last larvae died. 
No. 54, the surviving larva pupated. 
No. 55, the old larva is dead and also one of the recently hatched ones. 
Tube No. ,35 was inoculated with {S. cerevisiuf). 
168 days from start. 
No. 54, the pupa died. 
No. 55, an adult $ was reared from the surviving larva in this tube. 
Experiment XVIII. 
Killed B. coli culture and iHanipidafrd milk. 
A tube of distilled water, to which -washed B. coli had been added, was sterihzed 
by heat, and a sterile larva introduced that had hatched out in peptone broth. 
After 13 days during which the larva made no progress, 1 c.c. of sterilized milk, 
the colloid particles of which were increased in size but not precipitated by a 
manipulation of its acidity, was added. No progress occurred within 3 days; the tube 
was inoculated from a tube of broth in which a certain species of bacterium had 
developed. (This culture was used because its speed of growth under the conditions 
of the experiment had allowed the larva in the tube to develop satisfactorily.) The 
larva at once commenced to grow, and pupated within 20 days, an adult emerging 
in due course. 
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