E. E. Atkin and A. Bacot 
519 
third Lnstar, and one had failed to complete its moult satisfactorily. Three larvae 
were stiU hving on the 88th day after the addition of the milk; two were hving in 
the same stage after 104 days and one after 124 days. Sterile water was then added 
to the tube to replace evaporation and the tube was inoculated from a B. coli 
culture; the violence of the growth, however, killed the larvae. (Eggs were added 
to the tube; they hatched almost immediately and the larvae were killed off within 
a day, showing that the death of the larvae which had lingered on for so long a time 
was not in all probability connected with their age.) 
Various egg hatching and larva feeding tests. 
Experiment XXI. 
Sterile tubes prepared of filtrate from a B. coli culture; B. coli filtrate and 
precipitated white of egg; B. coli filtrate and milk in which the casein particles 
had been enlarged; autolyzed yeast extract in distilled water; peptone water; and 
distilled water. 
Eggs sterihzed by Method b were pipetted into each tube. 
No. of eggs 
hatched in 
Tubes 2J hours 
20 hours 48 hours 
96 hours 
1 
2 
3 
All hatched* 
All* larvae growing rapidly 
1 
1 
1 
All hatched* 
AH* larvae growing rapidly 
Filtrate of 
1 
0 
1 
All hatched* 
All* larvae growing rapidly 
B. coli cul- ^ 
1 
0 
0 
1 
All* larvae growing rapidly 
ture 
1 
0 
0 
0 
2, hatching became general 
and a mould appeared 
1 
0 
0* 
Used for special test, see Experiment IV, 
page 603. 
Filtrate of 
B. coli and 
1 
0 
1 
All hatched* 
Larvae growing rapidly 
precipitated 
1 
0 
0 
All hatched* 
Larvae growing rapidly 
white of egg 
Filtrate of 
B. coli and 
1 
0 
1 
All hatched* 
Larvae growing rapidly 
treated milk 
Autolyzed ' 
extract of 
1 
0 
0 
2 - 
brewers’ 
1 
0 
0 
0 
- 
No change 
yeast, in dis¬ 
1 
0 
0 
0 , 
tilled water , 
Peptone 
1 
1 
1 
1 
No change 
water 
1 
0 
0 
0 
1 
1 
1 
6 
6 
10 
1 
1 
3 
3 
6 
Distilled 
1 
0 
2 1 
water 
1 
0 
2 
Used in a 
special test; see Experiment IV, 
1 
0 
2 
page 603. 
1 
0 
1 ; 
* 
Tested for sterility—all proved to be infected. 
