524 
Stegomyia fasciata 
Eggs were used that had had time to incubate, but had not dried; these were well 
washed in three changes of clean tap water, put on to clean filter paper and then 
dried; they were subsequently sterilized by washing in weak -5 % lysol, then in 2 %, 
then in 1 %, and then in sterilized distilled water; 9 or 10 eggs were transferred 
by Pasteur pipette to the following tubes: 
Distilled water, Nos. 1 to 6. 
Distilled water with 1 c.c. of sterile B. coli filtrate ( = 1 in 6), Nos. 7 to 12. 
Beef broth (without peptone or salt). Nos. 13 to 18. 
Beef broth with 1 c.c. of sterile B. coli filtrate ( = 1 in 6), Nos. 19 to 24. 
No. of 
Hatching 
after 24 
After 
Media 
tube 
hours 
days 
1 
0 
0 
2 
0 
0 
Distilled water 
3 
4 
1 
0 
1 
0 
5 
0 
0 
6 
0 
0 
7 
0 
0 
Distilled water and 
8 
0 
0 
filtrate of B. coli 
!) 
2 
All* 
(= D.W. 6 c.c., F. ' 
10 
0 
All* 
1 c.c.) 
11 
3 
All* 
12 
0 
3 
13 
2 
All* 
14 
1 
1 
Beef broth without 
15 
0 
1 
sa.lt or peptone ) 
16 
0 
1 
17 
0 
0 
18 
1 
1 
19 
0 
0 
20 
0 
2 
Beef broth with fil¬ 
21 
0 
1 
trate of B. coli added^ 
22 
0 
7* 
( = B.B.6c.c.,F. Ic.e.) 
23 
0 
0 
24 
1 
1 
Remarks after 5 days 
The single larva which emerged 
did not grow. In one or two 
other tubes eggs uncapped, but 
the larvae died without quit¬ 
ting the shell. 
Sterility broke down after 24 
hours in Nos. 9, 10 and 11. and 
the larvae which emerged made 
rapid progress. 
Heavy growth of bacteria, larvae 
already in fouith instar. 
I.arva makes no progress. 
Larva makes scarcely any pro¬ 
gress. 
Larva makes no progress. 
Larva makes slight progress. 
Larvae show no progres.-^. 
Larva shows no progress. 
Heavy bacterial growth, larvae 
are already in fourth instar; 
remaining eggs failed to hatch. 
Larva shows no progress. 
On the 6th day tube No. 15 was inoculated from No. 13; all the eggs had hatched 
by the following morning. 
After the 1th day. 
Distilled water and Filtrate. There was a distinct difference as regards the pro¬ 
gress of the larvae in the infected tubes 9, 10 and 11; while the larvae in 9 and 11 
were on a parity as to size, those in 10 were slightly but definitely smaller. Sub- 
* Infected tubes. 
