E. E. Atkin and A. Bacot 
527 
After 49 days. 
Distilled water. No change. 
Distilled, water and Filtrate. No. 7, these larvae are now in their third and fourth 
instars. 
No. 8, 4 adults have been reared (within 21 days); there still remain 1 pupa and 
1 full-grown larva in the tube. 
No. 9, all the larvae but one are dead. 
No. 10, 1 adult has been reared; there are still 4 full-grown larvae in the tube. 
No. 12, the only larva is dead. 
Beef broth. No. 14, no change. 
No. 16, 1 has died; the 3 survivors have made no appreciable progress. 
No. 17, the larva in its second instar has died, but 2 more eggs have hatched; 
the remaining larva is still in its third instar. 
No. 18, 2 of the larvae in first instar have died. 
Beef broth and Filtrate. No. 19, no change. 
No. 20, 1 of the larvae has died. 
Nos. 21, 23 and 24, no change. 
After 50 days. 
Beef broth and Filtrate. Tube No. 19 was inoculated from a culture of B. coli 
on the 50th day; 8 or 9 eggs hatched within a few hr'n>«. 
After 60 days. 
Beef broth and Filtrate. About -5 c.c. of a broth culture of B. roli was added to 
tube No. 21 on the 60th day. A number of dormant eggs hatched within 10 to 15 
minutes. 
After 69 days. 
Distilled water. No change. 
Distilled water and Filtrate. No. 7, 1 adult reared; 1 died in pupal stage; 3 fourth 
instar larvae still living. A slow growing surface mould is present. 
No. 8, 2 more adults reared (6 in all). 
Nos. 9, 10 and 12, no change. 
Beef broth. No. 14, another larva has died; the 2 survivors appear very feeble. 
No. 16, all the larvae are dead. 
No. 17, 1 of the 3 larvae is dead. 
No. 18, 1 of the 2 larvae is dead. 
Beef broth and Filtrate. No. 19, 5 adults reared, 1 larva died when full grown. 
(Note these eggs were dormant for 50 days and the adults were reared within 
20 days of inoculation of the tube with B. coli.) 
No. 20, 1 larva has died. 
No. 21, the larvae which hatched in this tube after its inoculation with B. coli 
were full grown within 7 days. 
No. 23, the second instar larva is deeid; another egg has hatched. 
No. 24, no change. 
