528 
Stegomyia fasciata 
After 78 days. 
Distilled water. No. 7, a second adult reared; 1 larva living, the others died in 
fourth instar. 
No. 9, the last larva has died. 
No. 10, a second adult reared; 3 living larvae remain. 
No. 12, no change. 
Beef broth. No. 14, both the larvae are dead. 
No. 17, both the larvae are dead. 
No. 18, the larva is dead. 
Beef broth and Filtrate. No. 20, no change. 
No. 21, 7 adults reared within 16 days of the hatching of the eggs. 
No. 23, no change. 
No. 24, the third instar larva is dead; only 1 of the younger larvae is hving, 
it is still in its first instar. 
After 87 days. 
Distilled water. No change. 
Distilled water and Filtrate. No. 7, 1 larva marking time; moulds are growing 
out of the mouths of the dead larvae. 
Nos. 10 and 12, no change. 
Beef broth. No change. 
Beef broth and Filtrate. No. 20, only 1 first instar larva is now living. 
No. 21, the last larva has died. 
Nos. 23 and 24, no change. 
After 100 days. 
Distilled ivater. Tubes 4, 5 and 6 were used for the following experiment in con¬ 
junction with Experiment XXIV, page 530. 
To tube No. 4 about 3 % of a hving culture of B. coli was added. 
„ No. 5 ,, 3 % of a sterile autolyzed extract of brewers’ yeast. 
,, No. 6 ,, 3 % of a killed culture (autoclaved) of B. coli. 
Result: No. 4, 1 hatched within 2 minutes; 2 more within 5 minutes; 2 more 
within 10 minutes. 
No. 5, 1 hatched within 15—20 minutes. 
No. b, 1 ,, ,, ,, ,, 
After an hour and a half: 
No. 4, 8 out of 10 eggs hatched. 
No. 5, 1 „ 9 „ 
No. 6, 1 „ 11 „ 
A small quantity of living yeast cells from a wort agar culture was then trans¬ 
ferred by platinum loop to tube No. 5 and the remaining 8 eggs hatched within 
15 minutes. 
About 3 % of a hving broth culture of B. coli was added to No. 6, and 1 egg 
hatched within 3 minutes, another followed within 5 minutes and 4 more within 
15 minutes = 6 out of 10. 
