E. E. Atkin and A. Bacot 
529 
After 110 days. 
Distilled water. Nos. 1, 2 and 3, no change. 
No. 4, larvae now in second instar. 
No. 5, ,, „ third and fourth instars. 
No. 6, „ „ second and third instars. 
The rapid growth of the larvae in tube No. 5 should be noticed in contrast to 
what happened with regard to tubes Nos. 7 and 8, distilled water and filtrate, after 
infection with yeasts and bacteria on the 28th day. Presumably this difference is 
due to the media in tube No. 7 having been unfavourable for the yeast. 
Distilled water and Filtrate. No. 7, a further development of moulds; the larva 
is dead. 
No. 10, all died as larvae with the exception of one which reached the pupal 
stage. 
No. 12, an egg had hatched. 
Beef broth. Nos. 14, 16, 17 and 18, all the larvae in these tubes are dead; 
possibly some living eggs remain dormant. 
Beef broth and Filtrate. Nos. 20, 23 and 24, all the larvae are dead; possibly 
some hving eggs are lying dormant. 
After 134 days. 
Distilled water. Nos. 1 and 3, small white moulds developed in the tubes. 
Nos. 4, 5 and 6, want of nutriment checked development; a few larvae are hving 
in their second and third instar but many are dead. 
Distilled water and Filtrate. No. 10, another adult reared. 
No. 12, another egg hatched; both the larvae which hatched after the 87th 
day and this one which hatched after the 110th day are in their first instar. 
Beef broth. No larvae are living in these tubes, but it is possible that there may 
be dormant eggs. 
Beef broth and Filtrate. In No. 23 an egg has hatched since the 110th day. 
After 156 days. 
Distilled water. Tube No. 2 was infected with B. coli without result; it was 
concluded that all the remaining eggs were dead. 
Nos. 4, 5 and 6, 1 larva was hving in No. 4 and 3 in No. 5; all wbi^ dead in 
No. 6. 
Distilled water and Filtrate. No. 12, only 1 larva hving. 
Beef broth. No. 14, tested with B. coli for dormant eggs; none hatched. 
No. 16, an egg haxi hatched since the 134th day. 
Nos. 17 and 18, a httle of a broth culture of B. coli was added; dormant eggs 
hatched in both tubes within 15 minutes. 
Beef broth and Filtrate. The larva which hatched out of a dormant egg in 
No. 23 is dead. 
An egg hatched in tube No. 20 after the 134th day. 
After 203 days. 
AU the larvae are dead except the one in distiUed water and filtrate No. 12; this 
is now in its fourth instar. One adult was reared in tube No. 5, distilled water. 
