E. E. Atkin and A. Bacot 
y.i] 
Steam heated: 
B. coli . 
S. aureus ... 
Killed with chloroform: 
B. coli 
Filtrate of B. coli 
No. of Tube 
... No. 7,6% added I 
No. 8, 6 % „ J 
... No. 9,6% „ I 
No. 10, 6 % „ J 
...No. 11, 6% „ I 
No. 12, 6 % „ / 
... No. 13, 6 % „ I 
No. 14, 6 % „ j 
Hatching commenced vvitliin 
5 minutes. 
No effect within 10 minutes. 
Hatehing commeneed within 
10 minutc.s. 
No effect within 10 minutes. 
After half-an-hour. 
Autoclaved cultures : 
Autolyzed yeast extract... Nos. 1 and 2, all or a high percentage hatched. 
B. coli ... ... ... Nos. 3 and 4, „ „ ,, „ 
S. aureus . ... Nos. 5 and 6 , „ „ „ „ 
Steam healed: 
B. coli 
S. aureus ... 
Killed by chloroform: 
B. coli 
Filtrate of B. coli... 
... Nos. 7 and 8, all or a high percentage hatched. 
... No. 9, only one or two hail hatched. 
No. 10, all or a high percentage had hatched. 
... Nos. 11 and 12, a large number but certainly not aU. 
... Nos. 13 and 14, no effect. 
After 18 hours. 
Autoclaved: 
Autolyzed yeast extract... 
B. coli ... . 
S. aureus ... 
Steam heated: 
B. coli 
S. aureus ... 
No.s. 1 and 2, all hatched. 
Nos. 3 and 4 „ 
Nos. 5 and 6 ,, 
Nos. 7 and 8 „ 
Nos. 9 and 10 ,, 
Killed by chloroform: 
B. coli ... ... ... Nos. 11 and 12 „ 
Filtrate: 
B. coli .Nos. 13 and 14, a good proportion but by no means 
all hatched. 
It 'is to be noted that considerable numbers of eggs hatched out during this 
period in some of the remaining 10 tubes to '.iihich no addition had been made. 
After 11 days. 
Notes regarding the progress of the larvae. 
Autoclaved: 
Autolyzed yeast extract... No. 1, infected bacterial growth; larvae in second 
and third instar, 2 in fourth. 
No. 2, sterile, larvae still in first instar; no pro¬ 
gress. 
Parasitology ix 
35 
