53-2 
Stegomyia fasciata 
B. coli 
S. aureus 
Steam heated 
B. coli 
S. aureus ... 
Killed bjj chloroform : 
B. coli 
Filtrate : 
B. coli 
No. of Tube 
.. No. 3, infected, larvae still in second instar; 
growing. 
No. 4, sterile, larvae still in first instar; no pro¬ 
gress. 
.. No. 5, apparently sterile, larvae still in first instar; 
no progress. 
No. 6, apparently sterile, larvae still in first instar; 
but growing. 
.. No. 7, ? infected ] Larvae growing in first and 
No. 8, ? infected f second instars. 
.. No. 9, infected, larvae mostly in second instar, 
growing. 
No. 10, sterile, larvae still in first instar; no progress. 
.. No. 11, infected, and a mould growing, larvae of 
varied growth, first to third instars. 
No. 12, sterile, larvae in first instar; no progress. 
No. 13, infected, aU eggs hatched, larvae in second 
or third instars; growing. 
No. 14, sterile, a number still dormant; larvae in 
first instar; no progress. 
Unfortunately time did not permit of sub-culture tests to clear up doubtful 
points as to infection of tubes. 
The suggestion conveyed by the number of breaksdown in sterility is that the 
eggs were imperfectly sterilized. 
After 35 days. 
Nine spare tubes of distilled water: many eggs had hatched in all these tubes. 
In three of them some colourless, fuzzy looking growth has occurred, probablj^ of 
fungoid origin. In six there are no signs of any growth; in a tube that was infected 
with B. coli a few larvae had reached their third instar. 
Autolyzed yeast extract... No. 1, not much further progress; some had died. 
2, 2 or 3 had reached the second instar, the rest 
were dead. 
3, larvae had reached the second or third instar; 
many were dead and moulds were growing, 
in many cases from the mouths of the dead 
larvae. 
4, larvae in second or third instars seemingly 
quite healthy; this tube is now infected. 
5, only 2 living larvae, 1 in first and 1 in fovnth 
instar; the latter was fuU grown; a sur¬ 
face mould was growing—probable bac¬ 
terial infection also. 
6, only 1 third instar larva was living; a mould 
had almost covered the surface of the tube. 
B. coli 
S. aureus 
No. 
No. 
No. 
No. 
No. 
