536 
Stegomyia fasciata 
Tube No. 7 (infected), larval growth had slowed down; none had yet reached 
their fourth instar. 
Autolyzed extract of brewers’ yeast: 
Tube No. 5 (sterile), larvae were all still in first instar. 
„ No. 2 (infected), larvae in various stages of growth in second and third 
instar. 
,, No. 9, all killed by vigour of bacterial growth. 
„ No. 10 (infected), in third and fourth instar. 
Living yeasts {S. cerevisiae): 
The larvae in these tubes had been given more yeasts. In the tubes where they 
were most numerous they were in third and fourth instar. In tubes Nos. 11 
and 12 where fewer larvae were present a few adidts had already been bred 
and the remainder were either full grown larvae or pupae. 
After 42 days. 
Watery extract of brewers’ yeast; 
Tube No. 1 (sterile), all were dead; none past second instar. 
„ No. 3 (sterile), 3 or 4 living in second instar, the rest dead in first instar. 
„ No. 8 (infected), nearly all dead in various stages. 
„ No. 7 (infected), all dead but one in its third instar. 
Autolyzed extract of brewers’ yeast; 
Tube No. 5 (sterile), a few had reached their second instar. 
„ No. 2 (infected), but httle, if any progress since 18th day. 
„ No. 10 (infected), 1 adult reared, 1 pupa, many fuU-fed larvae. 
Living yeasts (S. cerevisiae). No food since 18th day; 
Tube No. 4, aU dead, but 1 in third instar. 
„ No. 6, all dead, but 1 in third instar. 
„ No. 11, 2 adults reared; remainder dead in various stages. 
„ No. 12, all dead, but 1 in fourth instar. 
The history of the larvae was not continued beyond this point. 
REFERENCES. 
^Aqramonte (1902). Berkeley’s Laboratory work with Mosquitoes. New York, 
1902. 112 pages, 16 figs. 
Bacot (1916). Yellow Fever Commission {West Africa). Reports on Questions 
connected with the Investigation of non-malarial Fevers in West Africa, ni. 
1-191. J. and A. Churchill, London. 
Boyce and Lewis (1910). The effect of Mosquito larvae upon drinking water. 
Ann. Trop. Med. and Parasitol. m. 591-594. 
Howard, Dyar and Knab (1912). The Mosquitoes of North and Central America 
and the West Indies. Carnegie Institute of Washington. I. 152 pp. 
McGregor (1916). Bulletin of Entomological Research, vn. 81-85. 
Mitchell (1907). Mosquito Life, p. 145. G. P. Putnam and Sons, New York and 
London, 1907. 
^ Both quotation and record are taken from Howard, Dyar and Knab (1912), Vol. i. 
p. 281. We have been unable to refer to this book in London. There is no reference 
to it in the Zoological Record. 
