T. Goodev and a. W. Weldings 
o4:j 
section of an intestinal ulcer showing Entamoeba liislohjlica which was 
kindly lent to us by Dr J. H. Ashw'orth, showed the cytoplasm of the 
amoeba stained blue with methylene-blue whilst the nucleus was stained 
pink with eosin. One generally thinks of nuclei as basophilous and 
not eosinophilous. 
Nuclear division must take place but so far we have not found any 
organisms which show any stage of the process. 
Vye have found one bi-nucleate amoeba which is shown in PI. XX, 
fig. 6, and on one film we were fortunate in obtaining a late stage in the 
fission of an amoeba, shown as a photomicrograph in PI. XX, fig. 7. 
So far, we have not been successful in finding either the cystic or 
the pre-cystic stage described and figured by Craig. 
Our measurements of the amoeba and the nucleus agree on the 
whole with those given by previous workers. One of the smallest 
forms we have found is that shown in Fig. 5 and measures 7-5 jx in its 
longer axis. The organisms vary from this up to about 25-27 /x when 
in an extended condition. We have not found any of the large forms 
mentioned by Smith and Barrett reaching 60/i. in length. The nucleus 
measures from about 2-5/.1 to 4p. in diameter. 
(c) The Food of Entamoeba gingivalis. 
The importance of determining the nature of the food of E. gingi¬ 
valis need not be emphasised here except to point out its relevancy to 
the question of the possible pathogenicity of the organism. 
Reference to the literature dealing with this subject yields no 
definite information on the point. Doflein (1911, p. 595) says that 
the amoeba feeds chiefly on leucocytes and bacteria, whilst Hartmann 
(1913, p. 641) says that the food consists of bacteria of the mouth 
cavity, but chiefly of leucocytes and leucocyte remains. 
These statements appear to be based largely on the remarks of 
von Prowazek and Leyden and Loewenthal on the subject. Prowazek 
(1904) says that E. hnccalis, which is undoubtedly the same organism 
as E.'gingivalis, lives among leucocyte masses, and takes in this food 
(presumably the leucocytes) by ectoplasmic engulfment. He goes on 
to describe the process of digestion, as revealed by the aid of neutral 
red, and the throwing out of the undigested nuclear remains. 
Leyden and Loewenthal (1905, p. 9), in speaking of the amoebae 
found by them on the surface of a cancer inside the mouth say that the 
food appears to consist chiefly of leucocytes, with whose remains they 
are frequently completely filled, whilst bacteria are seldom found in 
