2H4 UretJn'fil Spiroc1i(iet<>sis 
of the urethral discharge to a tube of nutrient bouillon (fig. I, 
62-64). 
Division then appeared to take place both transversely and 
longitudinally, but owing to the rapidity with which the patient 
recovered the observations made were not so numerous as could have 
been wished and I do not feel justified in making any dogmatic state¬ 
ment. 
Degeneration forms. In a tube of nutrient bouillon inoculated with 
the urethral discharge and kept in an incubator at 37° C. spirochaetes 
were still recognisable on the fifth day although no living ones had 
been seen since the second day. A number of these dead parasites 
showed the peculiar degeneration forms that have sometimes been 
described as “cysts”; that is to say large round cyst-like bodies 
had formed in them, generally at one end (see fig. I, 65 and 66). These 
cysts appeared to be empty, and were no doubt the result of plasmolysis 
(c/. Kindle, 1911). 
The Intracellular Phase of the Spirochaete. 
The discharge from the patient contained a great number of 
epithelial scales derived from the lining of the urethra. Some of these 
scales had a fairly healthy appearance, some seemed to be mere empty 
shells, and others were invaded by spirochaetes. 
A large proportion of epithelial scales contained minute rounded 
or oval granules which I believe to have been coccoid bodies. Some 
of the scales contained only a few, others contained great masses of 
them. Similar granules were found free in the pus of the discharge. 
These bodies showed up as clear refractile spots in fresh preparations 
and after fixation stained well with the usual reagents but were 
decolourised by Gram’s method. They were of about the same diameter 
as the coccoid bodies seen in the free spirochaetes and resembled small 
micrococci, but no such organism grew in any of the cultures made 
from the discharge. 
Other epithelial scales contained spirochaetes. In fresh specimens 
these cells were a striking feature since many of them were literally 
packed with exceedingly active parasites. When these scales were 
carefully examined the spirochaetes were seen to be collected at the 
periphery whilst in the middle was the nucleus surrounded by a larger 
or smaller core of granular cytoplasm in which coccoid bodies were 
usually abundant (fig. II). The spirochaetes were very active and 
