THE QUEEN-BEE. 
17 
on virgin queens, and on those with 
whose history he was not acquainted 
from their origin, and which perhaps 
had been impregnated unknown to him. 
Impressed with this idea, he under¬ 
took a new method of observation, not 
t 
on queens fortuitously taken from the 
hive; but on females decidedly in a 
virgin state, and whose history he 
knew from the moment they left the 
cell. 
From a great number of hives, he 
removed all the reigning females, and 
substituted for each, a queen taken 
at the moment of her birth. The 
hives were divided into two classes. 
From the first, all the males, both 
large and small were removed, and 
