G. H. F. Nuttall 
57 
0-5 % solution being injected subcutaneously into Calf 8 (see protocol 
of Exp. I) before the parasites appeared in the animal’s blood, Koch’s 
“blue bodies” having, however, been found in gland smears coincidently 
with the onset of fever. The administration of this dye did not retard 
the appearance of the parasites in the blood. The dye was tried because 
it had been found by me to exert an effect on Piroplasma canis {Parasi¬ 
tology, II. p. 415). 
I a. Sodium salicylate (10 %) + Sodium hicarhonate (1 %) in 
100 c.c. of NaCl solution v/as subsequently injected intravenously into 
Calf 8 on three days, beginning on the second day after the appearance 
of the parasites in the blood, but the result was negative. 
II-III. Tryposafrol (according to Prof. Brieger and Dr Krause, 
kindly supplied by the Aktien-Gesellschaft fiir Anilin Fabrikation, 
Berlin). This dye was given to two calves. In Exp. II it was given 
in daily doses ranging from 0-25 to 3 grammes dissolved in sugared 
water which the animal drank. Treatment began on the fifth day after 
the onset of fever and for six days before the parasites appeared in the 
animal’s blood, and continued daily up to and including the day of death. 
The number of parasites in the blood remained small but they showed 
a definite increase in spite of treatment. In Exp. Ill the calf received 
the same treatment, the dose of tryposafrol being 0-25, rising to 1-5 
grammes daily; treatment began on the day after the first parasites 
appeared in the animal’s blood and was maintained to the end. The 
number of parasitized corpuscles steadily increased in spite of treatment. 
(See protocol on p. 63.) This dye was tried because of the claims 
made by Brieger and Krause that it exerted a curative effect in piro- 
plasmosis of cattle and in trypanosomiasis. Our experiments with 
tryposafrol in relation to piroplasmosis and trypanosomiasis will be 
reported upon in a future paper. 
IV. Creosote 0-2 c.c. + Oleum copaivae 3-7 c.c. in 3 % solution 
injected intravenously in seven daily doses of 5 c.c. beginning on the 
third day of fever and three days before the appearance of parasites 
in the animal’s blood. No effect was exerted by the treatment (see 
protocol, p. 64). Creosote was chosen as a representative of antiseptic 
drugs belonging to the volatile aromatic series, being more powerfully 
antiseptic than carbolic acid and possessing greater penetrating power. 
The copaiva was added as a representative of the less irritant volatile 
oils with antiseptic properties. 
