E. Hindle and Lajos Gozony 
233 
distilled water at 37° C., in exactly the same way as the albumin, the 
liquid both within and without the capsule being covered with a layer 
of toluol as before. From each flask, 10 c.c. of the dialysate is then 
mixed with 0-2 c.c. of a 1 per cent, solution of Ninhydrin, and the 
mixture boiled in a test-tube for one minute. In every case a deep 
blue colour should be developed and if the intensity of the shade is not 
the same in all the tubes it is a sign that the capsules are not of equal 
permeability. Those capsules which only give a slight reaction must 
be discarded, as they are too dense to be employed. 
The capsules which have passed both these tests are washed once 
more in running water for 24 hours, then in boiling distilled water for 
30 seconds, and may be preserved in chloroform-water covered with a 
layer of toluol. 
When making a test, about 0-5 gm. of the prepared organ is placed 
in a capsule (a) and mixed with either 0-5, TO, or T5 c.c. of the serum 
to be examined. In another capsule (b) is placed an equal volume of 
serum alone, whilst in a third capsule (c) is placed an equal volume of 
inactivated serum^ mixed with 0-5 gm. of the prepared organ. Each 
capsule is then inserted in a flask containing 20 c.c. of sterilized distilled 
water and the surface of all the liquids covered with a layer of toluol. 
Then dialysis is allowed to continue at 37° C. for about 16 hours. 
The dialysates are tested for the presence of peptones by means of 
the Ninhydrin reaction, 0-2 c.c. of a 1 per cent, solution of Ninhydrin 
being added to 10 c.c. of each dialysate and the mixture boiled for 
exactly one minute. If the controls, h and c, both remain uncoloured 
whilst the dialysate of organ and serum gives a positive reaction, indicated 
by the development of a blue colour, it is a sign that the serum contains 
ferments which can digest the substance of the particular organ em¬ 
ployed. If the controls are slightly coloured, whilst the organ and 
serum is deeply coloured, so that there is a marked difference between 
them, the reaction is also positive ; on the other hand, should there be 
a slight difference between the colour of the organ and serum and the 
controls it is better to repeat the test employing only 0-5 or 1 c.c. serum, 
in order to bring out any differences more distinctly. After testing 
it is well to wash the capsules at once for 24 hours in running water 
followed by 30 seconds boiling in distilled water, as in this way they 
can be used four or five times before becoming too dense. 
Inactivated by heating at 60° C. for half-an-hour. 
Para.sitology vn 
16 
