E. Hindle and Lajos Gozony 
'23d 
gradually increase in number until the animal dies, the total duration 
of the disease from first putting on infected ticks to the death of the 
animal rarely exceeding one month. 
The pathological lesions observed at autopsy in a calf dead of East 
Coast Fever are relatively insignificant, practically none of the organs 
being markedly affected, and therefore this seemed a very suitable 
disease to study by means of Abderhalden’s method. 
Experiment 1. On March 7th, 1914, 50 infected nymphs of Rhijyi- 
cephalus appendiculatus were placed on a calf. On March 19th, it first 
showed febrile symptoms, parasites appeared in the peripheral blood 
five days later, and finally, on April 2nd. it was killed when moribund. 
On March 27th, when 12-8 per cent, of the corpuscles contained one or 
more parasites, a small quantity of the calf’s blood was collected and 
the serum tested for the presence of ferments against the following 
organs from a normal calf. 
In every case T5 c.c. of the serum from the infected calf was mixed 
with about 0-5 gm. of the organ (prepared as described above) and the 
mixture dialysed for about 16 hours at 37° C. 
As a control the serum from a normal calf was mixed with corre¬ 
sponding amounts of the same organs. 
The results were as follows, a positive or negative reaction being 
indicated by a -f- or — sign respectively : 
+ 1-5C.C. 
Organ employed infected serum 
Brain + 
Kidney - 
Liver + 
Lymphatic gland + 
Lung -f 
Pancreas + 
Spleen + 
Suprarenal gland + 
Thyroid ,, + 
Thymus „ + 
Control (serum alone) 
Control „ 
+ 1‘Sc.c. 
normal serum 
On April 2nd, when the calf was killed, all the organs were found 
to contain infarcts with Theileria parva present. Moreover, quite 
40 per cent, of the red blood corpuscles contained one or more 
parasites. The organs of this calf were prepared in the usual manner 
and employed for another series of tests with the calf’s own serum. 
16—2 
