236 
Ahderhalden's Reaction 
The results were as follows : 
+1-5 c.c. 
+inactive 
Organ employed 
infected serum 
infected serum 
Infected Brain 
+ 
- 
,, Diaphragm 
+ 
- 
„ Heart muscle 
+ 
- 
Lymphatic gland 
- 
- 
,, Lung 
- 
- 
,, Muscle 
-r 
- 
,, Pancreas 
+ 
- 
,, Spleen 
+ 
- 
,, Suprarenal gland 
+ 
- 
„ Thymus 
1- 
- 
„ Thyroid 
+ 
- 
Control (serum alone) 
- 
- 
9J ?S 
— 
— 
In addition, the red blood corpuscles were isolated from about one 
litre of this calf’s blood and after being well washed with normal salt 
solution were cytolized by means of digitoxin in order to isolate the 
parasites. After cytolysis was complete the parasites were washed 
free from haemoglobin and part of them were coagulated by mixing 
with egg albumin and boiling. The resulting coagulum was mixed 
with infected serum and dialysed, when a strongly positive reaction 
was obtained with the dialysate. 
From the above results it will be seen that the blood of a calf infected 
with East Coast Fever, contains ferments which can dissolve the albu¬ 
min of almost all organs, as well as the isolated parasites. 
Agglutination and complement fixation tests were also tried with 
other lots of the isolated parasites, but with uniformly negative results. 
Experiment 2. On April 30th, 100 infected nymphs {R. appendi- 
cidatus) were fed on a calf. On May 12th it first showed a rise in tem¬ 
perature and was bled and its serum tested for the presence of ferments. 
Six days later (May 18th), one day after parasites were first seen in the 
peripheral circulation, it was again bled and the serum tested as before, 
whilst yet a third series of tests were made with the blood of this animal 
on May 25th, when at least 30 per cent, of the corpuscles were infected. 
The animal died of East Coast Fever the same night. 
In every case 1 c.c. of the calf’s serum was mixed with about 0-5 gm. 
of the organ to be employed, and in most cases the organs used had 
been obtained from the calf of Experiment 1, and therefore contained 
parasites. 
As a control, some of the organs were mixed with inactivated serum 
as well as active. 
