238 
Ahde^'lialden's Reaction 
the serum contained so much haemoglobin that the reactions obtained 
in most of the experiments were positive. 
Experiment 1. A dog was inoculated with infected blood on April 
20th. Seven days later parasites appeared in its blood and on April 
30th it was killed, and its serum and organs used for the following tests : 
Organs 
Reaction 
Brain (from Babesia dog) -r 1 c.c. infected serum 
- 
Liver ,, 
+ 1 c.c. „ „ 
+ 
Pancreas „ „ 
+ 1 C.o. „ „ 
- 
Kidney 
+ 1 c.c. „ „ 
- 
I-ung 
+ 1 c.c. „ „ 
- 
Heart Muscle „ 
+ 1 c.c. 
- 
Control (serum alone) 
9i 99 
1 c.c. ,, ,, 
1 c.c. 
— 
This experiment, therefore, indicates that destruction of hver tissue 
has already commenced, but that the other organs are as yet unaffected. 
Experiment 2. A dog 
was inoculated with infected blood on June 
8th. Five days later parasites appeared in its blood and on June 15th, 
when it was heavily infected, the dog was killed and its blood tested 
with the following organs. In order to minimise 
the effect of the 
slight amount of haemoglobin present in the serum 
, only 0-5 c.c. was 
employed in each case. 
Organ 
Result 
Liver 
-I-0-5 c.c. active serum 
+ 
Brain 
+ 0 5 c.c. ,, 
“T 
Pancreas 
+ 0-5 c.c. „ 
+ 
Heart 
+ 0-5 c.c. .. „ 
+ 
Lung 
+ 0-5 c.c. „ „ 
+ 
Liver 
+ 0-5 c.c. inactivated serum 
- 
Heart 
+ 0-5 c.c. 
- 
Brain 
+ 0-5 c.c. „ „ 
- 
Control (serum alone) 
0-5 c.c. active serum 
— 
99 99 
0-5 c.c. ,. „ 
- 
The results of this experiment suggest that at a late stage of infec¬ 
tion not only is the liver affected, but also the other organs of the body. 
In both experiments the results agree with the pathology of this 
disease. The liver is especially affected, as evidenced by the appear¬ 
ance of jaundice in the majority of infected dogs, whilst at later stages 
of the disease, Graham-Smith (1905) has shown that extensive lesions 
may be observed in practically all the organs. 
