A. Porter 
325 
The flagellar end is rather blunt (Fig. 6). The posterior or aflagellar 
end is more pointed in some specimens (Figs. 7, 9), but is not markedly 
so in others (Figs. 6, 8). The nucleus exhibits the same two types as 
are seen in the pre-flagellates, namely, those with the chromatin dis¬ 
tributed in granules (Figs. 6, 10) and those with it concentrated as a 
karyosome (Figs. 5, 7). In forms about to divide, the nucleus is 
always granular in character and the karyosome is not seen. 
C. Post-jlagellate forms (Figs. 11-14). These resemble the pre- 
flagellates and are about the same size, but have somewhat thicker 
11 12 13 14 
Figs. 11-14. Herpetomonas patellce. Development of tlie post-flagellate form, x 3000. 
cyst walls. The nucleus most frequently shows homogeneous chromatin 
(Figs. 11-14), but occasionally a karyosome is present. The blepharo- 
plast usually is near the nucleus (Figs. 12-14); in a few specimens it 
was superimposed. 
The post-flagellate form of H. jMteJlae is derived from the flagellate 
by the retrogression and absorption of the flagellum (Fig. 11), acconin 
panied by the gradual concentration of the body cytoplasm around the 
nucleus. The wall at first is gelatinous and swells slightly if warm 
distilled water be added to the preparation. No swelling occurs in 
preparations made with physiological salt solution at room temperature. 
The gelatinous cyst wall gradually contracts and forms a varnish-like 
sheath in the final resting condition (Figs. 12-14). 
Multiplication. 
Multiplication occurs by binary fission of either the pre-flagellate 
(Fig. 3) or flagellate (Figs. 15-17) parasites. The division of the pre- 
flagellate (Fig. 3) is initiated by that of the blepharoplast. The nucleus 
gradually constricts into two. The cytoplasm segregates to either 
side of the body so that a continuous vacuole appears from end to end 
of the pre-flagellate. A cleft is next seen near the blepharoplast and 
gradually extends backwards to the posterior end. Slight movements 
of the body occur. No division' rosettes of the pre-flagellates of 
H. patellae were found. 
