380 
THE NUCLEAR CHANGES IN AGRIPPINA BONA 
STRICKLAND C 
By C. STBICKLAND, M.A., B.C. 
Travelling Medical Entomologist, Federated Malag States. 
{From the Malaria Bureau, Kuala Fumpur, F.M.S.) 
Lewin^ has recently described the account which I gave of the 
nuclear changes and sporulation of Agrippina hona as a remarkable 
narrative. 
His own description, however, differs but shghtly from mine, as the 
following table will show. 
My account 
1. The karyosome consists of a close-wound skein 
of chromatin in the young trophozoite. 
2. The nuclear “spherules” do not become 
chromatinised. 
3. In the cyst when the partition between the 
sporonts has disappeared the nucleus can 
no longer be seen, the membrane goes, the 
sphertdes go, and the karyosomatic band 
goes. 
4. The details of the differentiation of the 
endoplasm into spores is very difficult to 
make out. Clear areas (sporocysts) loom 
up and resolve themselves into spores. 
Lewin's account 
There is no evidence of this. 
They become chromatic^, but 
finally in the cyst achromatic 
again. 
The nuclear membrane disappears, 
the spherules go, and only a 
little chromatin remains in the 
tropho-nucleus. 
Mitosis occurs, gametes are formed 
which conjugate to form sporo¬ 
cysts and spores. 
These are the main differences in the accounts and it is therefore 
difficult to see why Lewin should have found mine so remarkable. 
Lewin certainly fills up a gap in my account by his description of the 
1 Strickland (1912). l\ircmtology, v, 97-108. 
2 Lewin (1913). Parasitology, w, 257-264. 
" “ Chromatinic ” according to Minchin. 
