Gr. H. F. Nuttall 
421 
23-33 days upon calves are maintained at ca. 15° C.; a $ remained 51 
days upon the scrotum of a ram maintained at ca. 20° C. The cJ remains 
upon the host long after the $ has dropped off gorged; 4 cj were observed 
to remain attached to the host for 4-8 months; they may die whilst 
remaining attached. Oviposition began 9-46 days after the gorged 
$ had abandoned the host; the process lasted 26-90 days and the 
$ survived 2-10 days after it was completed. About the same number 
of eggs are laid as in the case of A. hebraeum. Out of 10 batches of 
eggs, laid by as many females, 8 failed to hatch (at ca. 15° C.) and 2 
hatched (at 30° C.), the larvae emerging 16-31 days after the first eggs 
in the batch were laid. Development is therefore markedly influenced 
by temperature. Attempts to raise the larvae failed. The life-history 
appears to be essentially similar to that of A. hebraeum. 
AMBLYOMMA SPLENDIDUM. 
Of this species only a single male has hitherto reached me in a living 
condition. The tick emerged in June and survived unfed for about 160 
days in a glass jar containing slightly dampened earth, the jar being 
placed at 14-19° C. in the dark. This is the same specimen which was 
described and illustrated in Parasitology, vi. p. 49, pi. vii. 
DERMACENTOR RETICULATUS. 
This species was successfrdly raised in Cambridge from two unfed 
adults (N. 2156, 1 S, 1 ?) collected on vegetation, in Pina, Spain, 
12. V. 1913, by Rev. Longin Navas, S.J., of the Colegio de Salvador, 
Saragossa, to whom my best thanks are due for sending them. 
[Marzinowski and Bielitzer (1909, pp. 19, 24) found D. reticulatus 
(determined by L. G. Neumann) upon all the horses suffering from 
piroplasmosis which they examined in Russia. The tick usually 
occurred in large numbers on the infected horses, and it was demon¬ 
strated that adult ticks, the progeny of females collected from 
infected horses and fed on rabbits in the larval and nymphal stages, 
were capable of infecting horses with Piroplasma caballi Nuttall. 
The Russian authors give a few data regarding the biology of the 
tick as follows: gorged $s, collected on 5-13 May from an infected 
horse, when maintained at room-temperature, began to oviposit 
after 2-3 days, laying “some hundreds” of eggs. Larvae emerged 
from the eggs 2-3 weeks after they were laid. An attempt to raise 
